Development of a real time diagnosis of lymph node metastasis by ultrasound and nanobubbles

2011 Fiscal Year Final Research Report

• Project/Area Number: 21650124
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Medical systems
• Research Institution: Tohoku University
• Principal Investigator: KODAMA Tetsuya 東北大学, 大学院・医工学研究科, 教授 (40271986)
• Co-Investigator(Kenkyū-buntansha): HAYASE Toshiyuki 東北大学, 流体科学研究所, 教授 (30135313)
• Project Period (FY): 2009 – 2011
• Keywords: がん

Research Abstract

The aim of the present study is to develop a real time diagnostic method of lymphatic metastasis less than 1cm in diameter. First we developed the color difference based on standard deviation (CDSD)method. Next we succeeded in extracting vascular structures in the tumors of less than 1 cm in diameter by high-frequency ultrasound (HF-US)system, nanobubbles (NBs), and the CDSD method. We applied the methodology to the lymphatic metastasis model, and found that it was applicable to early diagnostic and treatment evaluation of lymphatic metastasis.

Research Products

• [Journal Article] Volumetric and angiogenic evaluation of antitumor effects with acoustic liposomes and high-frequency ultrasound (2011)
  • Author(s): Kodama T, Tomita N, Yagishita Y, Horie S, Funamoto K, Hayase T, Sakamoto M, Mori S
  • Journal Title: Cancer Research Volume: 71(22) Pages: 6957-6964
  • Peer Reviewed
• [Journal Article] Investigating the effect of polymeric approaches on circulation time and physical properties of nanobubbles (2011)
  • Author(s): du Toit LC, Govender T, Pillay V, Choonara YE, Kodama T
  • Journal Title: Pharmaceutical Research Volume: 28 Pages: 494-504
  • Peer Reviewed
• [Journal Article] Morphological study on acoustic liposome using transmission electron microscopy (2010)
  • Author(s): Kodama T, Tomita N, Horie S, Sax N, Iwasaki H, Suzuki R, Maruyama K, Mori S, Fukumoto M
  • Journal Title: Journal of Electron Microscopy Volume: 59(3) Pages: 187-196
  • Peer Reviewed
• [Journal Article] Cavitation bubbles mediated molecular delivery during sonoporation (2009)
  • Author(s): Kodama T
  • Journal Title: Journal of Biomechanical Science and Engineering Volume: 4 Pages: 124-140
  • Peer Reviewed
• [Journal Article] A novel strategy utilizing ultrasound for antigen delivery in dendritic cell-based cancer immunotherapy (2009)
  • Author(s): Suzuki R, Oda Y, Utoguchi N, Namai E, Taira Y, Okada N, Kadowaki N, Kodama T, Tachibana K, Maruyama K
  • Volume: 133
  • Pages: 198-205
  • Peer Reviewed
• [Presentation] Characteristics of the flow to the efferent lymphatic vessel upon increased pressure in the groin lymph node (2011)
  • Author(s): Hatakeyama Y, Kodama T
  • Organizer: Joint Research Program with Korea
  • Place of Presentation: Jeju University
  • Year and Date: 20110926-29
• [Presentation] Study of a highly accurate analysis technique with high frequency ultrasound (2011)
  • Author(s): Sato S, Kodama T
  • Organizer: Joint Research Program with Korea
  • Place of Presentation: Jeju University
  • Year and Date: 20110926-29
• [Presentation] Development of an exogeneous molecular delivery system into the lymph node via lymphatic vessel (2011)
  • Author(s): Kato S, Kodama T
  • Organizer: Joint Research Program with Korea
  • Place of Presentation: eju University
  • Year and Date: 20110926-29
• [Presentation] 鼠径リンパ節内の圧力上昇にともなう輸出リンパ管への流出特性 (2011)
  • Author(s): 畠山友梨子, 李麗, サックスニコラ, 阪本真弥, 森士朗, 小玉哲也
  • Organizer: 第70回日本癌学会学術総会予稿集127頁
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] Volumetric and angiogenetic imaging system by using nanobubbles and high-frequency ultrasound for evaluation of the antitumor effect by cisplatin (2010)
  • Author(s): Yagishita Y, Takata Y, Ohki K, Miyashita H, Morikawa H, Sakamoto M, Mori S, Kawamura H, Kodama T
  • Organizer: The 5th International Symposium on Medical, Bio-and Nano-Electronics
  • Place of Presentation: Sendai, Japan(Book of Abstract)
  • Year and Date: 20100224-25
• [Presentation] Four-dimensional high-frequency ultrasound imaging system for early detection of lymph node micro-metastasis (2010)
  • Author(s): Li L, Horie S, Rui CHEN, Watanabe Y, Baba T, Sax N, Sakamoto M, Mori S, Takahashi S, Kodama T
  • Organizer: The 5th International Symposium on Medical, Bio-and Nano-Electronics
  • Place of Presentation: Sendai, Japan(Book of Abstract)
  • Year and Date: 20100224-25
• [Presentation] TEM observation and analysis of echogenic nano-bubbles (2010)
  • Author(s): Sax N, Horie S, Li L, Chen R, Watanabe Y, Mori S, Kodama T
  • Organizer: The 5th International Symposium on Medical, Bio-and Nano-Electronics
  • Place of Presentation: Sendai, Japan(Book of Abstract)
  • Year and Date: 20100224-25
• [Presentation] Ultrasound molecular imaging of lymph node metastasis with nano/microbubbles. International Symposium of AIDS and Tuberculosis (ISAT2010) (2010)
  • Author(s): Li L, Chen R, Horie S, Watanabe Y, Baba T, Sax N, Sakamoto M, Mori S, Takahashi S, Kodama T
  • Organizer: International Symposium of AIDS and Tuberculosis (ISAT2010)
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100113-14
• [Presentation] Physical characterization of acoustic liposomes (2010)
  • Author(s): Sax N, Horie S, Li L, Chen R, Watanabe Y, Mori S, Kodama T
  • Organizer: International Symposium of AIDS and Tuberculosis (ISAT2010)
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100113-14
• [Presentation] 音響性リポソームの減衰特性における実験的かつ論理的解析 (2010)
  • Author(s): 小島貴則, 藤川重雄, 小玉哲也
  • Organizer: 日本超音波医学会東北地方会第40回学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-26
• [Presentation] マイクロバブルと高周波超音波を用いた三次元イメージング法による転移リンパ節の血管密度の評価 (2010)
  • Author(s): 佐藤祥太, 李麗, 柳下陽子, 阪本真弥, 高橋昭喜, 森士朗, 小玉哲也
  • Organizer: 日本超音波医学会東北地方会第40回学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-26
• [Presentation] Developement and characterization of echogenic pogylated liposomes (2010)
  • Author(s): Sax N, Horie S, Li L, Chen R , Watanabe Y, Mori S, Sakamoto M, Kodama T
  • Organizer: 第26回日本DDS学会学術集会プログラム予稿集288頁
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2010-06-17
• [Presentation] ナノバブルと高周波超音波を用いた腫瘍血管構築法の開発 (2010)
  • Author(s): 柳下陽子, 森士朗, 李麗, 渡邊夕紀子, 堀江佐知子, 陳鋭, 高田陽子, 川村仁, 森川秀広, 小玉哲也
  • Organizer: 第26回日本DDS学会学術集会プログラム予稿集299頁
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2010-06-17
• [Presentation] Three-dimentional High-Frequency Ultrasound Imaging for early diagnosis of lymph node metastasis Combined with Microbubbles (2009)
  • Author(s): Li L, Horie S, Chen R, Watanabe Y, Sakamoto M, Mori S, Takahashi S, Kodama T
  • Organizer: The 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering.
  • Place of Presentation: Engineering Auditorium, National University Of Singapore, Singapore
  • Year and Date: 20091221-22
• [Presentation] 口腔癌治療のためのナノバブルと超音波を用いた抗腫瘍効果増強法に関する検討 (2009)
  • Author(s): 宮下仁, 森士朗, 高田陽子, 大木宏介, 川村仁, 森川秀広, 小玉哲也
  • Organizer: 日本口腔外科学会雑誌2009年総会特別号Vol.55. 91頁
  • Year and Date: 20091009-11
• [Presentation] Molecular delivery system by nanobubbles and ultrasound. International Symposium on HIV/AIDS, Medicinal Plants and Geophagia, Advancing the frontiers of Research on HIV/AIDS (2009)
  • Author(s): Kodama T
  • Organizer: Medicinal Plants and Geophagia at Walter Sisulu University Health Resource Centre Mthatha
  • Place of Presentation: Eastern Cape, South Africa
  • Year and Date: 20090722-24
• [Presentation] ナノバブルを用いた超音波によるリンパ節転移早期診断システムの研究開発 (2009)
  • Author(s): 李麗, 堀江佐知子, 陳鋭, 渡邊夕紀子, 阪本真弥, 森士朗, 高橋昭喜, 小玉哲也
  • Organizer: 第25回日本DDS学会. プログラム予稿集363頁
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 20090703-07
• [Presentation] ナノバブルと高周波超音波を用いた血管抽出法の開発 (2009)
  • Author(s): 小玉哲也
  • Organizer: 第25回日本DDS学会. プログラム予稿集252頁
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 20090703-07
• [Remarks]
  • URL: http://www.ecei.tohoku.ac.jp/kodama/index.html