Custom-shaping system for bone regeneration by seeding marrow stromal cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21659259
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: KAWAKITA Atsuo 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 共同研究員 (40338083)
• Co-Investigator(Kenkyū-buntansha): UMEZAWA Akihiro 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486) ; TOYAMA Yoshiaki 慶應義塾大学, 医学部, 病院長(教授) (40129549) ; KATO Tatsuo 国立成育医療研究センター(研究所), 総長 (50051826) ; TOYODA Masashi (地独)東京都健康長寿医療センター, 研究副部長 (50392486)
• Project Period (FY): 2009 – 2011
• Keywords: 成育医学

Research Abstract

Cytokines have been shown to promote regeneration in tissues. In addition, scaffold is required to maintain tissue organization and keep cytokines at target sites. In this study, we generated biodegradable hybrid sheets(poly-DL-lactic-co-glycolic acid(PLGA) sheets) with collagen microsponges. PLGA skeleton facilitates the formation of the hybrid sheets into desired shapes, and the collagen microsponges promote adhesion of the chimeric protein that is composed of cytokines and collagen binding domain of fibronectin. The chimeric protein clearly functioned in vivo, implying that PLGA sheets with chimeric cytokines show promise for use as a tool for the development of therapeutic applications.