Development of ultrasound and bubble-liposome-mediated drug delivery to ocular tissues

2011 Fiscal Year Final Research Report

• Project/Area Number: 21659400
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kagoshima University
• Principal Investigator: SAKAMOTO Taiji 鹿児島大学, 医歯学総合研究科, 教授 (10235179)
• Research Collaborator: TACHIBANA Katsuro 福岡大学, 医学部, 教授 ; MARUYAMA Kazuo 帝京大学, 薬学部, 教授
• Project Period (FY): 2009 – 2011
• Keywords: 超音波 / ナノバブル / リポソーム製剤

Research Abstract

We developed a novel method to deliver genes or drugs to ocular tissues using ultrasound and bubble-liposome. GFP cDNA embedded in bubble-liposome was injected in the vitreous followed by irradiation of ultrasound. GFP was strongly expressed by retina where ultrasound was irradiated. Tissue damage was minimal. We also developed a new ultrasound probe for that.

Research Products

• [Journal Article] Selective gene transfer to the retina using intravitreal ultrasound irradiation (2012)
  • Author(s): Sonoda S, Tachibana K, Yamashita T, Shirasawa M, Terasaki H, Uchino E, Suzuki R, Maruyama K, Sakamoto T.
  • Journal Title: J Ophthalmol Volume: Volume2012 Pages: 5
  • DOI: doi:10.1155/2012/412752
  • Peer Reviewed
• [Journal Article] Combination of ultrasound and bubble liposome enhance the effect of doxorubicin and inhibit murine osteosarcoma growth (2011)
  • Author(s): Ueno Y, Sonoda S, Suzuki R, Yokouchi M, Kawasoe Y, Tachibana K, Maruyama K, Sakamoto T, Komiya S.
  • Journal Title: Cancer Biol Ther Volume: 12(4) Pages: 270-277
  • URL: http://dx.doi.org/10.4161/cbt.12.4.16259
  • Peer Reviewed
• [Journal Article] Atypical presentation of acute macular neuroretinopathy with tiny parafoveal reddish-brown lesions (2011)
  • Author(s): Matsuo Y, Uemura A, Nakano T, Inoue M, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol Volume: 55(4) Pages: 362-364
  • DOI: DOI:10.1007/s10384-011-0028-0
  • Peer Reviewed
• [Journal Article] Ocular surface molecule after transconjunctival vitrectomy (2011)
  • Author(s): Fujita A, Uchino E, Otsuka H, Arimura N, Noda Y, Ishibashi T, Sakamoto T.
  • Journal Title: Br J Ophthalmol Volume: 95(3) Pages: 419-423
  • Peer Reviewed
• [Journal Article] Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment (2010)
  • Author(s): Otsuka H, Arimura N, Sonoda S, Nakamura M, Hashiguchi T, Maruyama I, Nakao S, Hafezi-Moghadam A, Sakamoto T.
  • Journal Title: Am J Pathol Volume: 177(5) Pages: 2268-2277
  • URL: http://dx.doi.org/10.2353/ajpath.2010.100134
  • Peer Reviewed
• [Journal Article] Combination therapy for retinal vein occlusion (2010)
  • Author(s): Shimura M, Yasuda K, Nakazawa T, Takeshita T, Shiono T, Sakamoto T.
  • Journal Title: Ophthalmology Volume: 117(9) Pages: 1858, 1858. e1-3
  • URL: http://dx.doi.org/10.1016/j.ophtha.2010.03.077
  • Peer Reviewed
• [Journal Article] Aniridia associated with aphakia and secondary glaucoma (2010)
  • Author(s): Terasaki H, Yamashita T, Tanaka M, Takahashi M, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol Volume: 54(5) Pages: 504-505
  • DOI: DOI:10.1007/s10384-010-0852-7
  • Peer Reviewed
• [Journal Article] Vitrectomy for exudative age-related m acular degeneration with vitreous hemorrh age (2010)
  • Author(s): Sakamoto T, Sheu SJ, Arimura N, Sames hima S, Shimura M, Uemura A, Kawano H, Wu TT, Kubota T, Sohma R, Noda Y.
  • Journal Title: Retina Volume: 30(6) Pages: 856-864
  • DOI: DOI:10.1097/IAE.0b013e3181c969cb
  • Peer Reviewed
• [Journal Article] Predictable signs of benign course of polypoidal choroidal vasculopathy : based upon the long-term observation of non-treated eyes (2010)
  • Author(s): Okubo A, Arimura N, Abematsu N, Sakamoto T.
  • Volume: 88(4)
  • Pages: e107-114
  • DOI: DOI:10.1111/j.1755-3768.2009.01850.x
  • Peer Reviewed
• [Journal Article] B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes (2010)
  • Author(s): Shrestha B, Hashiguchi T, Ito T, Miura N, Takenouchi K, Oyama Y, Kawahara K, Tancharoen S, Ki-I Y, Arimura N, Yoshinaga N, Noma S, Shrestha C, Nitanda T, Kitajima S, Arimura K, Sato M, Sakamoto T, Maruyama I.
  • Journal Title: J Immunol Volume: 184(9) Pages: 4819-4826
  • DOI: DOI:10.4049/jimmunol.0903063
  • Peer Reviewed
• [Journal Article] Steroid-induced Glaucoma in Children With Acute Lymphoblastic Leukemia : A Possible Complication (2010)
  • Author(s): Yamashita T, Kodama Y, Tanaka M, Yamakiri K, Kawano Y, Sakamoto T.
  • Journal Title: J Glaucoma Volume: 19(3) Pages: 188-190
  • DOI: DOI:10.1097/IJG.0b013e3181af321d
  • Peer Reviewed
• [Journal Article] Panretinal photocoagulation induces pro-inflammatory cytokines and macular thickening in high-risk proliferative diabetic retinopathy (2009)
  • Author(s): Shimura M, Yasuda K, Nakazawa T, Abe T, Shiono T, Iida T, Sakamoto T, Nishida K.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 247(12) Pages: 1617-1624
  • DOI: DOI:10.1007/s00417-009-1147-x
  • Peer Reviewed
• [Journal Article] Sonothrombolysis for intraocular fibrin formation in an animal model (2009)
  • Author(s): Yamashita T, Ohtsuka H, Arimura N, Sonoda S, Kato C, Ushimaru K, Hara N, Tachibana K, Sakamoto T.
  • Journal Title: Ultrasound Med Biol Volume: 35(11) Pages: 1845-1853
  • URL: http://dx.doi.org/10.1016/j.ultrasmedbio.2009.05.023
  • Peer Reviewed
• [Presentation] Early detection of macular hole closure in gas-filled eyes, Special Invited Lecture (2011)
  • Author(s): Sakamoto T.
  • Organizer: 2011 International ongress of the Korean Retina Society
  • Place of Presentation: St Mary's hospital, Seoul, Korea
  • Year and Date: 2011-06-18
• [Presentation] SDF1 is essential for photoreceptor protection in retinal detachment (2011)
  • Author(s): Sakamoto T.
  • Organizer: 2011 International ongress of the Korean Retina Society
  • Place of Presentation: St Mary's hospital, Seoul, Korea
  • Year and Date: 2011-06-18
• [Presentation] Optic disc swelling in Vogt-Ko yanagi-Harada disease (2011)
  • Author(s): Nakao K, Abematsu N, Mizushima Y, Sakamoto T.
  • Organizer: Joint Congress of SOE/AAO
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2011-06-04
• [Presentation] Assay For Establishment Of Highly Polarized Porcine Retinal Pigment Epithelial Cells (2011)
  • Author(s): Shozo Sonoda, Makoto Shirasawa, Hiroki Otsuka, Toshio Hisatomi, Noboru Arimura, Yasushi Sonoda, Taiji Sakamoto
  • Organizer: ARVO2011
  • Place of Presentation: Fort Lauderdale・U. S. A
  • Year and Date: 2011-05-01
• [Presentation] Chromovitrectomy (2010)
  • Author(s): Taiji Sakamoto
  • Organizer: Yale Eye Center Clinical Conference Series
  • Place of Presentation: U. S. A(New Haven.)
  • Year and Date: 2010-10-22
• [Presentation] Biology of Vitreous Cavity : Roles of Hyalocytes (2010)
  • Author(s): Taiji Sakamoto
  • Organizer: Yale Eye Center Clinical Conference Series
  • Place of Presentation: U. S. A(New Haven.)
  • Year and Date: 2010-10-21
• [Presentation] Non-steroidal anti-inflammatory drug(NSAID) in exudative AMD (2010)
  • Author(s): 坂本泰二
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2010-04-16
• [Presentation] Effect of Intravitreal Triamcinolone Acetonide for Polypoidal Choroidal Vasculopathy in Japanese (2009)
  • Author(s): Yoshinaga N, Okubo A, Arimura N, Abematsu N, Sakamoto T.
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, U. S. A
  • Year and Date: 2009-05-03
• [Presentation] Vitreous SDF-1 and other cytokines in rhegmatogenous retinal detachment (2009)
  • Author(s): Otsuka H, Arimura N, Yamakiri K, Hashiguchi T, Maruyama I, Sakamoto T.
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, U. S. A
  • Year and Date: 2009-05-03
• [Patent(Industrial Property Rights)] 血管新生抑制剤 (2011)
  • Inventor(s): 小戝健一郎,坂本泰二,上笹貫太郎
  • Industrial Property Rights Holder: 鹿児島大学
  • Industrial Property Number: 特許、国内出願特願2011-510400
  • Filing Date: 2011-10-20
• [Patent(Industrial Property Rights)] 血管新生抑制剤 (2010)
  • Inventor(s): 小戝健一郎,坂本泰二,上笹貫太郎
  • Industrial Property Rights Holder: 鹿児島大学
  • Industrial Property Number: 特許、国際出願PCT/JP2010/057735
  • Filing Date: 2010-04-23
  • Overseas