Strategy for treatment of periodontitis with regulating exocytosis using functional bionano particle

2011 Fiscal Year Final Research Report

• Project/Area Number: 21659436
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: National Center for Geriatrics and Gerontology
• Principal Investigator: MATSUSHITA Kenji 独立行政法人国立長寿医療研究センター, 口腔疾患研究部, 部長 (90253898)
• Co-Investigator(Kenkyū-buntansha): EGUCHI Takanori 独立行政法人国立長寿医療研究センター, 口腔疾患研究部, 室長 (20457229)
• Project Period (FY): 2009 – 2011
• Keywords: 免疫 / 感染 / 炎症 / 歯周病学 / 生体分子設計 / 低分子Gタンパク質 / 上皮機能 / 細胞骨格タンパク質 / エンドサイト-シス / 細胞内輸送

Research Abstract

We explored factors for intracellular vesicle trafficking in gingival epithelial cells and examined a possibility of the regulation. A small G-protein Rab5 and the associated protein vinculin were identified as important factors for vesicle trafficking in the cells. Rab5 and ICAM-1 were associated with uptake of Porphyromonas gingivals by the cells. Furthermore, we clarified that E-selectin is important for regulating endothelial exocytosis by stimulation with P. gingivalis.

Research Products

• [Journal Article] Valproic acid increases susceptibility to endotoxin shock through enhanced release of HMGB1 (2011)
  • Author(s): Sugiura S, Ishihara Y, Komatsu T, Hagiwara M, Tanigawa N, Kato Y, Mizutani H, Kawahara K, Maruyama I, Noguchi T, Matsushita K
  • Journal Title: Shock Volume: 36 Pages: 494-500
  • DOI: DOI:10.1097/SHK.0b013e31822f7e58
  • Peer Reviewed
• [Journal Article] Complete pulp regeneration after pulpectomy by transplantation of CD105+stem cells with SDF-1 (2011)
  • Author(s): Iohara K, Imabayashi K, Ishizaka R, Watanabe A, Nabekura J, Ito M, Matsushita K, Nakamura H, Nakashima M
  • Journal Title: Tissue Eng Part Volume: 17 Pages: 1911-1920
  • DOI: DOI:10.1089/ten.tea.2010.0615
  • Peer Reviewed
• [Journal Article] Dental Pulp Derived CD31-/CD146-Side Population Stem/Progenitor Cells Enhance Recovery of Focal Cerebral Ischemia in Rats (2011)
  • Author(s): Sugiyama M, Iohara K, Wakita H, Hattori H, Ueda M, Matsushita K, Nakashima M
  • Journal Title: Tissue Eng Part A Volume: 17 Pages: 1303-1311
  • DOI: DOI:10.1089/ten.tea.2010.0306
  • Peer Reviewed
• [Journal Article] Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function (2011)
  • Author(s): Kanno Y, Ishisaki A, Nakajima K, Nishihara T, Toyoshima T, Okada K, Ueshima S, Matsushita K, Matsuo O
  • Journal Title: J Biol Chem Volume: 286 Pages: 8952-8960
  • DOI: DOI:10.1074/jbc.M110.152181
  • Peer Reviewed
• [Journal Article] orroniside cinnamic acid conjugate as an anti-inflammatoryagent (2011)
  • Author(s): Takeda Y, Tanigawa N, Sunghwa F, Ninomiya M, Hagiwara M, Matsushita K, Koketsu M
  • Journal Title: J Biol Chem Volume: 20 Pages: 4855-4857
  • DOI: DOI:10.1016/j.bmcl.2010.06.095
  • Peer Reviewed
• [Journal Article] 高齢者住民における保有歯数と認知機能 (2010)
  • Author(s): 今井剛、西永正典、中村知子、奥宮清人、松林公蔵、土居義典、松下健二
  • Journal Title: 愛院大歯誌 Volume: 48 Pages: 59-66
  • Peer Reviewed
• [Journal Article] IL-4 alters expression patterns of torage components of vascular endothelial cell-specific granules through STAT6-and SOCS-1-dependent mechanisms (2009)
  • Author(s): Inomata M, Into T, Nakashima M, Noguchi T, Matsushita K
  • Journal Title: Mol Immuno Volume: 48 Pages: 2080-2089
  • DOI: DOI:10.1016/j.molimm.2009.02.015
  • Peer Reviewed
• [Journal Article] Degradation of vascular endothelial thrombomodulin by arginine and lysine-specific cysteine proteases from Porphyromonas gingivalis (2009)
  • Author(s): Inomata M, Ishihara Y, Matsuyama T, Imamura T, Maruyama I, Noguchi T, Matsushita K
  • Journal Title: J Periodonto Volume: 80 Pages: 1511-1517
  • URL: http://www.joponline.org/doi/abs/10.1902/jop.2009.090114?url_ver=Z39.88-2003 & rfr_id=ori:rid:crossref.org & rfr_dat=cr_pub%3dpubmed
  • Peer Reviewed
• [Journal Article] Aldosterone activates endothelial exocytosis (2009)
  • Author(s): Jeong Y, Chaupin DF, Matsushita K, Yamakuchi M, Cameron S, Morell CN, Lowenstein CJ
  • Journal Title: Proc Natl Acad Sci USA Volume: 106 Pages: 3782-3787
  • URL: http://www.pnas.org/content/106/10/3782.long
  • Peer Reviewed
• [Journal Article] Regeneration of dental pulp after pulpotomy by transplantation of CD31-/CD146-side population cells from a canine tooth (2009)
  • Author(s): Iohara K, Zheng L, Ito M, Ishizaka R, Nakamura H, Into T, Matsushita K, and Nakashima M
  • Journal Title: Regen Med Volume: 4 Pages: 377-385
  • DOI: DOI:10.2217/rme.09.5
  • Peer Reviewed
• [Journal Article] Matrix metalloproteinase-3 accelerates wound healing following dental pulp injury (2009)
  • Author(s): Zheng L, Amano K, Iohara K, Ito M, Imabayashi K, Into T, Matsushita K, Nakamura H, and Nakashima M
  • Journal Title: Am J Pathol Volume: 175 Pages: 1905-1914
  • URL: http://www.sciencedirect.com/science/article/pii/S0002944010607018
  • Peer Reviewed
• [Journal Article] 高齢者の残存歯数と認知機能の関連性 (2009)
  • Author(s): 今井剛、西永正典、松下健二
  • Journal Title: 鹿児島大学医学雑誌 Volume: 61 Pages: 47-51
  • Peer Reviewed
• [Presentation] Porphyromonas gingivalisジンジパインによるヒト歯肉上皮細胞のアレルギー誘導性サイトカインの発現機構 (2011)
  • Author(s): 多田浩之,島内英俊,松下健二
  • Organizer: 第17回日本エンドトキシン・自然免疫研究会
  • Place of Presentation: 西宮
  • Year and Date: 2011-12-10
• [Presentation] Rab5とvinculin依存的なエンドサイトーシスの解析 (2011)
  • Author(s): 萩原真,杉浦進介,小松寿明,加藤佳子,多田浩之,磯田竜太郎,石田直之,小林かおる,谷川順美,松下健二
  • Organizer: 34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-10
• [Presentation] 老年期、衰退期を想定した歯科医学・医療とQOL (2011)
  • Author(s): 松下健二
  • Organizer: 第12回抗加齢歯科医学研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-18
• [Presentation] 歯周病関連細菌orphyromonas gingivalisの歯肉上皮細胞への侵入機構の解明 (2011)
  • Author(s): 加藤佳子,杉浦進介,小松寿明,石原裕一,野口俊英,松下健二
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-27
• [Presentation] エンドサイトーシスが関与する誤嚥性肺炎の発症機序に関する研究 (2011)
  • Author(s): 萩原真,小松寿明,杉浦進介,加藤佳子,谷川順美,松下健二
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-14
• [Presentation] A MULTIDISCIPLINARY COMMUNITY CARE PROGRAM FOCUSING ON ORAL HEALTH (2011)
  • Author(s): Sakashita R, Watanabe K, Hamada M, Matsushita K, Nishitani M, Nishihira T
  • Organizer: The International Council of Nurses
  • Place of Presentation: Malta
  • Year and Date: 2011-05-05
• [Presentation] Morroniside derivative regulates E-selectin expression in human endothelial cells (2011)
  • Author(s): Tanigawa N, Takeda Y, Sunghwa F, Ninomiya M, Hagiwara M, Koketsu M, Matsushita K
  • Organizer: The 4th International Symposium for Interface Oral Health Scienc
  • Place of Presentation: Sendai, Miyagi, Japan
  • Year and Date: 2011-03-07
• [Presentation] バルプロ酸はHMGB1の能動放出を誘導して、エンドトキシンショックに対する感受性を高める (2010)
  • Author(s): 杉浦進介,石原裕一,小松寿明,萩原真,谷川順美,水谷大樹,加藤佳子,江口傑徳,野口俊英,松下健二
  • Organizer: 第16回日本エンドトキシン・自然免疫研究会
  • Place of Presentation: 奈良
  • Year and Date: 2010-11-13
• [Presentation] E-selectin mediates Porphyromonas gingivalis adherence to endothelial cells (2010)
  • Author(s): Komatsu T, Nagano K, Sugiura S, Hagiwara M, Tanigawa N, Eguchi T, Yoshimura F, Furuichi Y, Matsushita K
  • Organizer: The 96th Annual Meeting of American Academy of Periodontology
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2010-10-31
• [Presentation] Valproic acid enhances innate immune response to LPS by HMGB1 release (2010)
  • Author(s): Sugiura S, Ishihara Y, Komatsu T, Hagiwara M, Tanigawa N, Eguchi T, Mizutani H, Matsushita K, Noguchi T
  • Organizer: The 96th Annual Meeting of American Academy of Periodontology
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2010-10-31
• [Presentation] オーラルヘルスプロモーションのこれから-口腔健康行動におけるコミュニティーチーム医療- (2010)
  • Author(s): 松下健二
  • Organizer: 日本健康心理学会
  • Place of Presentation: 千葉
  • Year and Date: 2010-09-11
• [Presentation] E-selectin mediates Porphyromonas gingivalis adherence to endothelial cells (2010)
  • Author(s): Komatsu T, Nagano K, Sugiura S, Hagiwara M, Tanigawa N, Eguchi T, Yoshimura F, Furuichi Y and Matsushita K
  • Organizer: 第8回血液・血管オルビス
  • Place of Presentation: 東京
  • Year and Date: 2010-08-21
• [Presentation] Valproic acid enhanced innate immune response induced by LPS through stimulating active release of HMGB1 (2010)
  • Author(s): Sugiura S, Ishihara Y, Komatsu T, Hagiwara M, Tanigawa N, Mizutani H, Eguchi T, Kawahara K, Maruyama I, Noguchi T and Matsushita K
  • Organizer: 第8回血液・血管オルビス
  • Place of Presentation: 東京
  • Year and Date: 2010-08-21
• [Presentation] 歯髄幹細胞を用いた抜髄後歯髄再生のタンパク化学的解析による証明 (2010)
  • Author(s): 今林貴代美、庵原耕一郎、石坂亮、江波久哲、松下健二、中村洋、中島美砂子
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-05
• [Presentation] 歯髄CD105陽性細胞を用いた抜髄後歯髄再生法の確立 (2010)
  • Author(s): 庵原耕一郎、石坂亮、今林貴代美、江場久哲、松下健二、中村洋、中島美砂
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-05
• [Presentation] MMP-3はイヌ一部性歯髄炎モデルにおいて歯髄治癒を促進する (2010)
  • Author(s): 江場久哲、中島美砂子、庵原耕一郎、松下健二、中田和彦、中村洋
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-05
• [Presentation] バルプロ酸(VPA)はERK 1/2の活性化を介してHigh Mobility Group Box 1(HMGB1)の産生を誘導する (2010)
  • Author(s): 杉浦進介、江口傑徳、萩原真、小松寿明、谷川順美、野口俊英、松下健二
  • Organizer: 第33回日本血栓止血学会学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-04-24
• [Presentation] Angiotensin II Activates Endothelial Exocytosis By Stimulating Superoxide Production (2010)
  • Author(s): Matsushita K, Morrell CN, Mason, EL O' Rourke B, Champion HCAnd Lowenstein CJ
  • Organizer: 第33回日本血栓止血学会学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-04-24
• [Presentation] 凝固第Xa因子の病態への関与とその制御-基礎研究者の立場から (2010)
  • Author(s): 松下健二
  • Organizer: 第3回Bayer Thrombosis Seminar
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-04-22
• [Presentation] MP3はクロマチンに局在し, CCNファミリー遺伝子を制御する (2009)
  • Author(s): 江口傑徳,河田かずみ,久保田聡,萩原真,小松寿明,杉浦進介,中島美砂子,滝川正春,松下健二
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 高齢化社会における歯学の使命と課題-老年期、衰退期を想定した歯科医療・医学とQOL-、DENTISTRY, QUO VADIS (2009)
  • Author(s): 松下健二
  • Organizer: フロネシスに基づいて
  • Place of Presentation: 東京
  • Year and Date: 2009-12-05
• [Presentation] 血管の詰りを予防する食品有用成分の探索-特に岐阜県特産イチゴに関して- (2009)
  • Author(s): 松下健二
  • Organizer: ぎふEBBFフォーラム2009
  • Place of Presentation: 各務原
  • Year and Date: 2009-12-02
• [Presentation] 高齢者の様々な口腔保健行動が口腔状態に及ぼす影響 (2009)
  • Author(s): 坂下玲子,桑原未代子,松下健二,佐藤拓一,安彦友希,三重幸恵,井上昌一
  • Organizer: 第58回日本口腔衛生学会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 2009-10-10
• [Presentation] エキソサイトーシス制御を応用した新しい血管病治療の戦略 (2009)
  • Author(s): 松下健二
  • Organizer: 日本杜仲研究会第4回定期大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-08-01
• [Presentation] E-selectinの新機能:感染の制御 (2009)
  • Author(s): 小松寿明,江口傑徳,杉浦進介,猪俣恵,古市保志,松下健二
  • Organizer: 日本歯科保存学会2009年度春季学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-11
• [Presentation] 新規炎症性サイトカインHMGB1のアセチル化制御薬による放出制御 (2009)
  • Author(s): 杉浦進介,江口傑徳,猪俣恵,小松寿明,野口俊英,松下健二
  • Organizer: 第52回日本歯周病学会春季学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-15
• [Presentation] Th2由来サイトカインはSTAT6を介して血管内皮細胞特異的な分泌顆粒の構成因子の発現量を変化させる (2009)
  • Author(s): 猪俣恵,引頭毅,石原裕一,松下健二,野口俊英
  • Organizer: 第52回日本歯周病学会春季学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-15
• [Presentation] Aldosterone activates endothelial exocytosis (2009)
  • Author(s): Jeong Y, Chaupin DF, Matsushita K, Yamakuchi M, Cameron S, Morell CN, Lowenstein CJ
  • Organizer: The ATVB 2009 Annual Conference
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2009-04-29
• [Presentation] Porphyromonas gingivalis is widely distributed in subgingival plaque biofilm of elderly people (2009)
  • Author(s): Abiko Y, Sato T, Matsushita K, Sakashita R, Takahashi N
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai, Miyagi
  • Year and Date: 2009-01-15
• [Book] Oral Health Science 2011 (2012)
  • Author(s): Tanigawa N, Takeda Y, Sunghwa F, Ninomiya M, Hagiwara M, Koketsu M, Matsushita K
  • Publisher: Springer
• [Book] Oral Health Science 2011 (2012)
  • Author(s): Nishihira T, Nishitani M, Sato T, Abiko Y, Matsushita K, Hamada M, Sakashita R
  • Publisher: Springer
  • URL: http://www.springerlink.com/content/h4u46l758g711k16/
• [Book] 歯周病と炎症 (2011)
  • Author(s): 松下健二
  • Publisher: メディカルレビュー社
• [Book] エンドトキシン研究14:バルプロ酸はHMGB1の能動放出を誘導して、エンドトキシンショックに対する感受性を高める (2011)
  • Author(s): 杉浦進介,石原裕一,小松寿明,萩原真,水谷大樹,加藤佳子,野口俊英,松下健二
  • Publisher: 日本エンドトキシン・自然免疫研究会
• [Book] Morroniside derivative regulates E-selectin expression in human endothelial cells (2011)
  • Author(s): Tanigawa N, Takeda Y, Sunghwa F, Ninomiya M, Hagiwara M, Koketsu M, Matsushita K
  • Total Pages: 161-163
• [Book] バルプロ酸はHMGB1の能動放出を誘導して、エンドトキシンショックに対する感受性を高める (2011)
  • Author(s): 杉浦進介,石原裕一,小松寿明,萩原真,水谷大樹,加藤佳子,野口俊英,松下健二
  • Total Pages: 57-60
  • Publisher: エンドトキシン研究
• [Book] 歯周病と炎症、The Bone (2011)
  • Author(s): 松下健二
  • Total Pages: 415-450
• [Remarks]
  • URL: http://www.ncgg.go.jp/department/odr/index.html