Analysis of the acetylation of SMC3

2012 Fiscal Year Final Research Report

• Project/Area Number: 21681025
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: The University of Tokyo (2011-2012); Tokyo Institute of Technology (2009-2010)
• Principal Investigator: KATOU Yuki 東京大学, 分子細胞生物学研究所, 助教 (50391917)
• Project Period (FY): 2009 – 2012
• Keywords: 染色体 / ゲノム機能 / 細胞周期

Research Abstract

To understand how “sister chromatid cohesin” is loading onto chromosome or establishin S phase 、I tried to analyze Eco1 using S.cerevisiae. Though I tried to know thedistribution of Eco1 using ChIP-chip technique first, it was hard to detect distributionclearly. Next I tried to make improvements of ChIP-chip or ChIP-seq technique. At lastwe made strains which has the region of Smc3 is replaced into humarized Smc3. This straincan detect by human acetylated Smc3 antibody. Finally using these strains and antibodywe can detect of the acetylation region of Smc3.

Research Products

• [Journal Article] Shirahige K, Labib K.Replisome stability at defective DNA replication forks is independent of S phase checkpoint kinases (2012)
  • Author(s): De Piccoli G, Katou Y , Itoh T, Nakato
  • Journal Title: Mol Cell Volume: 45(5) Pages: 696-704
  • Peer Reviewed
• [Journal Article] EndogenousDNA replication stress results inexpansion of dNTP pools and a mutator phenotype (2012)
  • Author(s): Davidson MB, KatouY , Keszthelyi A,Sing TL, Xia T, Ou J, Vaisica JA,Thevakumaran N, Marjavaara L, Myers CL,Chabes A, Shirahige K, Brown GW
  • Journal Title: EMBO J Volume: 31(4) Pages: 895-907
  • Peer Reviewed
• [Journal Article] Shirahige K, Araki H.Origin association of Sld3,Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing. (2011)
  • Author(s): Tanaka S, Nakato R, Katou Y
  • Journal Title: Curr Biol Volume: 21(24) Pages: 2055-63
  • Peer Reviewed
• [Journal Article] ATP hydrolysis is required for relocating cohesin from sites occupied (2011)
  • Author(s): Hu B*, Itoh T*, Mishra A,KatohY , Chan KL, Upcher W, Godlee C, Roig MB, Shirahige K^#,Nasmyth K^#.
  • Journal Title: Curr Biol Volume: 21(1) Pages: 12-24
  • Peer Reviewed
• [Journal Article] Involvement of a chromatin remodelingcomplex in damage tolerance during DNA (2009)
  • Author(s): Falbo KB*, Alabert C*, K a t o u Y * , Wu S,Han J, Wehr T, Xiao J, He X, Zhang Z, ShiY, Shirahige K, Pasero P, Shen X.
  • Journal Title: Nat Struct .Mol Biol Volume: 16(11) Pages: 1167-72
  • Peer Reviewed
• [Journal Article] Tof1,and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks (2009)
  • Author(s): Bando M*, Katou Y *, Komata M, Tanaka H,Itoh T, Sutani T, Shirahige K. Csm3
  • Journal Title: J Biol Chem Volume: 284(49) Pages: 34355-65
  • Peer Reviewed
• [Journal Article] Ctf4 coordinates the progression of helicase and DNA polymerase alpha. Genes (2009)
  • Author(s): Tanaka H*, Katou Y * , Yagura M, Saitoh K, Itoh T, Araki H, Bando M, Shirahige K.
  • Journal Title: Cells Volume: 14(7) Pages: 807-20
  • Peer Reviewed
• [Presentation] Genome-wide Dynamic change of Cohesin and replication in yeast. (2012)
  • Author(s): Yuki Katou, Ryuichiro Nakato,Katsuhiko Shirahige
  • Organizer: The 13th International Conference on Systems Biology
  • Place of Presentation: in Toronto
  • Year and Date: 20120819-23
• [Presentation] 発表タイトル:次世代シーケンサーを使用した姉妹染色分体間の接着形成に必要な因子の解析 (2010)
  • Author(s): 加藤由起、伊藤武彦、白髭克彦
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: in Kobe Japan
  • Year and Date: 20101207-10
• [Presentation] Csm3,Tof1,and Mrc1 form a Heterotrimeric Mediator Complex that Associates with DNA Replication Forks (2009)
  • Author(s): 加藤由起、田中宏和,白髭克彦
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: in
  • Year and Date: 20091209-12
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/chromosomeinformatics/