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2011 Fiscal Year Final Research Report

Comprehensive functional screening and application of cytochrome P450 from basidiomycetes

Research Project

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Project/Area Number 21688013
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Wood science
Research InstitutionKyushu University

Principal Investigator

ICHINOSE Hirofumi  九州大学, 大学院・農学研究院, 助教 (00432948)

Project Period (FY) 2009 – 2011
Keywords木材腐朽担子菌・シトクロムP450・リグニン
Research Abstract

We explored the molecular diversity of basidiomycetous cytochrome P450 and isolated 304 genes encoding full-length cDNA. The isolated P450 genes were transformed into Saccharomyces cerevisiae to construct a functional library. Using the functional library, the catalytic potentials of P450s against a wide variety of compounds were elucidated. A functionomic survey allowed the discovery of novel catalytic potentials. An increased compilation of P450 functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.

  • Research Products

    (28 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (17 results) Remarks (1 results)

  • [Journal Article] 2. Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta2012

    • Author(s)
      Ide M, Ichinose H, Wariishi H.
    • Journal Title

      Wariishi H. Arch Microbiol

      Volume: 194(4) Pages: 243-53

    • Peer Reviewed
  • [Journal Article] Heterologous expression and mechanistic investigation of a fungal cytochrome P450(CYP5150A2): Involvement of alternative redox partners2012

    • Author(s)
      Ichinose H., Wariishi H.
    • Journal Title

      Arch Biochem Biophys

      Volume: 518(1) Pages: 8-15

    • Peer Reviewed
  • [Journal Article] Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae. Nazir KHMNH2011

    • Author(s)
      Nazir KHMNH, Ichinose H, Wariishi H.
    • Journal Title

      Appl Environ Microbiol

      Volume: 77(9) Pages: 3147-50

    • Peer Reviewed
  • [Journal Article] Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium : Involvement of versatile monooxygenase2011

    • Author(s)
      Hirosue S, Tazaki M, Hiratsuka N, Yanai S, Kabumoto H, Shinkyo R, Arisawa A, Sakaki T, Tsunekawa H, Johdo O, Ichinose H, Wariishi H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 407(1) Pages: 118-23

    • Peer Reviewed
  • [Journal Article] Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium2010

    • Author(s)
      Chigu NL, Hirosue S, Nakamura C, Teramoto H, Ichinose H, Wariishi H
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 87(5) Pages: 1907-16

    • Peer Reviewed
  • [Journal Article] Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae2010

    • Author(s)
      Nazir KHMNH, Ichinose H, Wariishi H.
    • Journal Title

      Arch Microbiol

      Volume: 192(5) Pages: 395-408

    • Peer Reviewed
  • [Journal Article] Metabolism of mono-and dichloro-dibenzop-dioxins by Phanerochaete chrysosporium cytochromes P4502010

    • Author(s)
      Kasai N, Ikushiro S, Shinkyo R, Yasuda K, Hirosue S, Arisawa A, Ichinose H, Wariishi H, Sakaki T
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 86(2) Pages: 773-80

    • Peer Reviewed
  • [Journal Article] Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete 0 5 10 15 20 0. 00 0. 02 0. 04 0. 06 0. 08 0. 10[ PBA](μM)! Absorbance! Absorbance Wavelength(nm) 400 550 0. 04 0. 02-0. 02 350 450 500-0.2010

    • Author(s)
      Kasai N, Ikushiro S, Hirosue S, Arisawa A, Ichinose H, Uchida Y, Wariishi H, Ohta M, Sakaki T
    • Journal Title

      J Biochem.

      Volume: 147(1) Pages: 117-25

    • Peer Reviewed
  • [Journal Article] 10. Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium2009

    • Author(s)
      Kasai N, Ikushiro S, Hirosue S, Arisawa A, Ichinose H, Wariishi H, Ohta M, Sakaki T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 387(1)] Pages: 103-8

    • Peer Reviewed
  • [Journal Article] Molecular and functional diversity of fungal cytochrome P450s.

    • Author(s)
      Ichinose H
    • Journal Title

      Biol. Pharm. Bull

      Volume: (in press)

    • Peer Reviewed
  • [Presentation] Molecular diversity and functional application of cytochrome P450 from basidiomycetes2011

    • Author(s)
      Ichinose H.
    • Organizer
      The 6th International Medicinal Mushrooms Conference
    • Place of Presentation
      Zagreb, Croatia
    • Year and Date
      2011-09-27
  • [Presentation] 真核微生物が有するシトクロムP450 :機能解明と高度利用2011

    • Author(s)
      一瀬博文
    • Organizer
      第84回日本生化学会大会(シンポジウム)
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
  • [Presentation] Functionomic survey and catalytic application of cytochrome P450 monooxygenases from fungi2011

    • Author(s)
      Ichinose H., Wariishi H.
    • Organizer
      17th International Conference on Cytochrome P450 Biochemistry, Biophysics and Structure
    • Place of Presentation
      Manchester, UK
    • Year and Date
      2011-06-26
  • [Presentation] Functional application of fungal cytochrome P450 for bioindustry2011

    • Author(s)
      Ichinose H.
    • Organizer
      33rd Symposium on Biotechnology for Fuels and Chemicals
    • Place of Presentation
      Seattle USA
    • Year and Date
      2011-05-02
  • [Presentation] 担子菌が有するシトクロムP450機能の解明と利用2011

    • Author(s)
      井手正迪,一瀬博文,割石博之
    • Organizer
      第61回日本木材学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-19
  • [Presentation] 真核微生物が有するシトクロムP450の機能スクリーニング2011

    • Author(s)
      眞田有規,一瀬博文,割石博之
    • Organizer
      第62回日本木材学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2011-03-15
  • [Presentation] Molecular and functional characterization of cytochromes P450 from the filamentous fungus Aspergillus oryzae2010

    • Author(s)
      Nazir KHMNH., Ichinose H., Wariishi H.
    • Organizer
      IMC9 The Biology of Fungi
    • Place of Presentation
      Edinburgh UK
    • Year and Date
      2010-08-01
  • [Presentation] Molecular diversity of cytochromes P450 from wood-rotting basidiomycetes2010

    • Author(s)
      Ide M., Ichinose H., Wariishi H.
    • Organizer
      IMC9 The Biology of Fungi
    • Place of Presentation
      Edinburgh UK
    • Year and Date
      2010-08-01
  • [Presentation] Functional characterization of cytochromes P450 from the filamentous fungus Aspergillus oryzae2010

    • Author(s)
      Nazir KHMN., Ichinose H., Wariishi H.
    • Organizer
      The 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
    • Place of Presentation
      Boston USA
    • Year and Date
      2010-03-20
  • [Presentation] Molecular identification and functional characterization of cytochromes P450 from wood-rotting fungi2010

    • Author(s)
      Ide M., Ichinose H., Wariishi H.
    • Organizer
      The 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnolog
    • Place of Presentation
      Boston USA
    • Year and Date
      2010-03-20
  • [Presentation] 褐色腐朽担子菌シトクロムP450のクローニングおよび異種発現2010

    • Author(s)
      井手正迪,一瀬博文,割石博之
    • Organizer
      第60回日本木材学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-03-17
  • [Presentation] Phanerochaete chrysosporiumが有するクロロペルオキシダーゼ遺伝子の単離および同定2010

    • Author(s)
      嶋岡隆行,一瀬博文,割石博之
    • Organizer
      第60回日本木材学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-03-17
  • [Presentation] Functional diversity and enzymatic application of cytochrome P450 from wood-rotting basidiomycetes2009

    • Author(s)
      Ichinose H., Hirosue H., Tazaki M., Arisawa A., Jodo O., Wariishi H.
    • Organizer
      Asia pacific biochemical engineering conference
    • Place of Presentation
      Kobe Japan
    • Year and Date
      2009-11-25
  • [Presentation] 木材腐朽担子菌におけるシトクロムP450分子種の多様性2009

    • Author(s)
      井手正迪,一瀬博文,割石博之
    • Organizer
      第54回リグニン討論会
    • Place of Presentation
      静岡
    • Year and Date
      2009-10-29
  • [Presentation] 15. Molecular characterization and isolation of cytochrome P450 genes from Aspergillus oryzae2009

    • Author(s)
      Nazir KHMN. H., Ichinose H., Wariishi H.
    • Organizer
      6th International Conference on Cytochrome P450
    • Place of Presentation
      Okinawa Japan
    • Year and Date
      2009-06-21
  • [Presentation] Gene expression profiling and enzymatic function of Phanerochaete chrysosporium cytochrome P450 involved in xenobiotic metabolism of aromatic compounds2009

    • Author(s)
      Chigu N. L., Ichinose H., Wariishi H.
    • Organizer
      16th International Conference on Cytochrome P450
    • Place of Presentation
      Okinawa Japan
    • Year and Date
      2009-06-21
  • [Presentation] Functional characterization of cytochrome P450 from the white-rot fungus Phanerochaete chrysosporium2009

    • Author(s)
      Ichinose H., Hirosue H., Tazaki H., Arisawa H., Jodo H., Wariishi H.
    • Organizer
      16th International Conference on Cytochrome P450
    • Place of Presentation
      Okinawa Japan
    • Year and Date
      2009-06-21
  • [Remarks] ホームページ等

    • URL

      http://brc.wood.agr.kyushu-u.ac.jp/madlab/

URL: 

Published: 2013-07-31  

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