2011 Fiscal Year Final Research Report
Comprehensive functional screening and application of cytochrome P450 from basidiomycetes
Project/Area Number |
21688013
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Wood science
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Research Institution | Kyushu University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Keywords | 木材腐朽担子菌・シトクロムP450・リグニン |
Research Abstract |
We explored the molecular diversity of basidiomycetous cytochrome P450 and isolated 304 genes encoding full-length cDNA. The isolated P450 genes were transformed into Saccharomyces cerevisiae to construct a functional library. Using the functional library, the catalytic potentials of P450s against a wide variety of compounds were elucidated. A functionomic survey allowed the discovery of novel catalytic potentials. An increased compilation of P450 functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.
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[Journal Article] Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium : Involvement of versatile monooxygenase2011
Author(s)
Hirosue S, Tazaki M, Hiratsuka N, Yanai S, Kabumoto H, Shinkyo R, Arisawa A, Sakaki T, Tsunekawa H, Johdo O, Ichinose H, Wariishi H
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Journal Title
Biochem Biophys Res Commun
Volume: 407(1)
Pages: 118-23
Peer Reviewed
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