2010 Fiscal Year Final Research Report

Accurate structural analysis of natively unfolded proteins by the solution X-ray scattering

Research Project

Project/Area Number	21700322
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Bioinformatics/Life informatics
Research Institution	Iwate Medical University (2010) Nagaoka University of Technology (2009)
Principal Investigator	SEKI Yasutaka Iwate Medical University, 薬学部, 講師 (30377220)
Project Period (FY)	2009 – 2010
Keywords	分子シミュレーション / 天然解鎖蛋白質 / 溶液X線散乱法
Research Abstract	A new method for generating polypeptide conformations has been developed for studying structural characteristics of unfolded proteins. Those of proteins in high-concentration denaturant solution were analyzed by comparing predictions from this method with results from solution X-ray scattering measurement. Analysis of the dependence of mean square radius on the number of residues and the shape of Kratky profiles for polypeptide chains has confirmed that a high-concentration denaturant solution serves as a good solvent. We found also that, for a conformational ensemble to reproduce experimental data, the probability that main-chain dihedral angles are in the α-region must be in the range of 20 to 40%.

(5 results)