Generation of transgenic mice expressing genetically-encoded fluorescent protein sensors for elucidation of odor information processing in the olfactory bulb

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700441
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MUTOH Hiroki The Institute of Physical and Chemical Research, 神経回路ダイナミクス研究チーム, 研究員 (60443040)
• Project Period (FY): 2009 – 2010
• Keywords: 嗅脳 / 膜電位センサー / 脳機能光学測定 / 遺伝子改変マウス / in vivo / 匂いの記憶・学習

Research Abstract

To carry out our project, we generated transgenic mice which are specifically expressed VSFPs in mitral/tufted cells. Current situation, we are screening transgenic mice lines by checking expression patter and function of VSFPs. In parallel, we demonstrated functional property of VSFPs in living animals by generating in utero electroporation. As a result, VSFPs is working in living mice and has sufficient sensitivity for monitoring neuronal activities to natural sensory input.

Research Products

• [Journal Article] Optogenetic monitoring of membrane potentials. (2011)
  • Author(s): Mutoh, H., Perron, A., Akemann, W., Iwamoto, Y., Knopfel, T.
  • Journal Title: Exp Physiol. 96 Pages: 13-18
  • Peer Reviewed
• [Journal Article] 膜電位感受性蛍光タンパク質による脳機能イメージング (2011)
  • Author(s): 武藤弘樹., Akemann, W., Knopfel, T.
  • Journal Title: Medical Bio. Vol.8, No 1 Pages: 24-31
• [Journal Article] 膜電位感受性蛍光タンパク質の開発と神経活動観察 (2011)
  • Author(s): 武藤弘樹., Knopfel, T.
  • Journal Title: BIO INDUSTRY. Vol.28, No 2 Pages: 18-25
• [Journal Article] Imaging brian electric signals with genetically targeted voltage-sensitive fluorecsent proteins. (2010)
  • Author(s): Akemann, W., Mutoh, H., Perron, A., Rossier, J., Knopfel, T.
  • Journal Title: Nat Methods.*These authors contributed equally to this work. 7 Pages: 643-649
  • Peer Reviewed
• [Journal Article] Second and third generation voltage-sensitive fluorescent proteins for monitoring membrane potential. (2009)
  • Author(s): Perron, A., Mutoh, H., Akemann, W., Gautam, SG., Dimitrov, D., Iwamoto, Y., Knopfel, T.
  • Journal Title: Front Mol Neurosci. 2
  • Peer Reviewed
• [Journal Article] Spectrally-resolved response properties of the three most advanced FRET based fluorescent Protein voltage probes. (2009)
  • Author(s): Mutoh, H., Perron, A., Dimitrov, D., Iwamoto, Y., Akemann, W., Chudakov, DM., Knopfel, T.
  • Journal Title: PLoS ONE.*These authors contributed equally to this work. 4 Pages: e4555
  • Peer Reviewed
• [Journal Article] Red-shifted voltage-sensitive fluorescent proteins. (2009)
  • Author(s): Perron, A., Mutoh, H., Launey, T., Knopfel, T.
  • Journal Title: Chem Biol.*These authors contributed equally to this work. 24 Pages: 1268-1277
  • Peer Reviewed
• [Journal Article] Effect of voltage sensitive fluorescent proteins on neuronal excitability. (2009)
  • Author(s): Akemann, W., Lundby, A., Mutoh, H., Knopfel, T.
  • Journal Title: Biophys J. 96 Pages: 3959-3976
  • Peer Reviewed
• [Journal Article] Charge movement of a voltage-sensitive fluorescent protein. (2009)
  • Author(s): Villalba-Galea, CA., Sandtner, W., Dimitrov, D., Mutoh, H., Knopfel, T., Bezanilla, E.
  • Journal Title: Biophys J. 96 Pages: 19-21
  • Peer Reviewed
• [Journal Article] Exploration of fluorescent protein voltage probes based on circularly permuted fluorescent proteins. (2009)
  • Author(s): Gautam, SG., Perron, A., Mutoh, H., Knopfel, T.
  • Journal Title: Front Neuroengineering. 2
  • Peer Reviewed
• [Journal Article] Optical measurement of mGluR1 conformational changes reveals fast activation, slow deactivation and sensitization. (2009)
  • Author(s): Marcaggi. P., Mutoh, H., Dimitrov, D., Knopfel, T.
  • Journal Title: Proc Nati Acad Sci USA. 106 Pages: 11388-11393
  • Peer Reviewed
• [Journal Article] Optical imaging of postsynaptic odor representation in the glomerular layer of the mouse olfactory bulb. (2009)
  • Author(s): Fletcher, ML., Masurkar, AV., Xing, J., Imamura, F., Xiong, W., Nagayama, S., Mutoh, H., Greer, CA., Knopfel, T., Chen, W.
  • Journal Title: J Neurophysiol. 102 Pages: 817-830
  • Peer Reviewed
• [Presentation] Voltage sensitive protein 2.3 : a novel tool to study sarcolemmal structure and electrical activity in mouse hearts. (2011)
  • Author(s): Chang Liao M., Mutoh H., Iwamoto Y., Raad N., Nikolaev V., Luther S., Lehnart S., Wagner S., Maier L., Stuhmer W., Knopfel T., Zimmerman W.
  • Organizer: Biophysics 55^<nd>
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 2011-03-09
• [Presentation] Characterization of second generation voltage-sensitive fluorescent proteins as genetically-encoded probes of membrane voltage of neurons in vitro. Soc. (2010)
  • Author(s): Akemann W., Mutoh H., Perron A., Dimitrov D., Knopfel T.
  • Organizer: Neurosci 40^<th>
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-11-17
• [Presentation] Characterization of second generation voltage-sensitive fluorescent proteins as genetically-encoded probes of membrane voltage of neurons in vivo. (2010)
  • Author(s): Mutoh H., Akemann W., Perron A., Dimitrov D., Knopfel T.
  • Organizer: Soc. Neurosci 40^<th>
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-11-17
• [Presentation] Red-shifted fluorescent reporter proteins for membrane potential. (2010)
  • Author(s): Perron A., Mutoh H., Launey T., Knopfel T.
  • Organizer: 2010 Canadian Neuroscience Meeting
  • Place of Presentation: Ottawa, Canada
  • Year and Date: 2010-05-16
• [Presentation] Optical recording of electrical activity of pyramidal neurons using a genetically-encoded voltage probe. (2010)
  • Author(s): Akemann W., Mutoh H., Perron A., Gautam S., Dimitrov D., Yoshida R., Shimogori T., Launey T., Knopfel T.
  • Organizer: Francis Crick Neuroscience Symposium
  • Place of Presentation: Suzhou, China
  • Year and Date: 2010-04-14
• [Presentation] Second and third generation voltage-sensitive fluorescent proteins. (2010)
  • Author(s): Akemann W., Mutoh H., Yoshida R., Shimogori T., Knopfel T.
  • Organizer: Biophysics 54^<nd>
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2010-02-02
• [Presentation] Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. (2009)
  • Author(s): Knopfel T ., Mutoh H., Perron A., Gautam S., Dimitrov D., Akemann W.
  • Organizer: Soc. Neurosci 39^<th>
  • Place of Presentation: Chicago, USA
  • Year and Date: 20091017-20091021
• [Presentation] Second and third generation voltage-sensitive fluorescent proteins. (2009)
  • Author(s): Perron A., Dimitrov D., Mutoh H., Yokoyama J., Knopfel T.
  • Organizer: Fluorescence 2009, Fluorescence in Biology An International Conference
  • Place of Presentation: Mumbai, India.
  • Year and Date: 20090316-20090319
• [Book] Membrane Potential Imaging in the Nervous System. Chapter 14 (2011)
  • Author(s): Jin, L., Mutoh, H., Knopfel, T., Cohen, LB., Hughes, T., Pieribone, VA., Isacoff, EY., Salzberg, BM., Baker, BJ.
  • Total Pages: 157-163
  • Publisher: Genetically encoded protein sensors of membrane potential. Springer.
• [Book] Imaging the Brain with Optical Methods. Chapter 2 (2010)
  • Author(s): Jin, L., Baker, BJ., Cohen, LB., Mutoh, H., Dimitrov, D., Perron, A., Iwamoto, Y., Isacoff, EY., Pieribone, VA., Hughes, T., Knopfel, T., Akemann, W.
  • Total Pages: 27-44
  • Publisher: Fluorescent sensors of membrane potential that are genetically encoded. Springer.
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