Validation of mutant mice that exhibit AD/HD like behavior as psychiatric-disease models

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700452
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FURUSE Tamio The Institute of Physical and Chemical Research, マウス表現型解析開発チーム, 開発研究員 (60392106)
• Project Period (FY): 2009 – 2010
• Keywords: AD / HD / ENU mutagenesis / Methylphenidate / 疾患モデル / 発達障害

Research Abstract

We screened mice that exhibit AD/HD like behavior in RIKEN ENU mutagenesis program. In the screening, we isolated 2 mutant lines, M-174 and M-1736, as candidates of AD/HD model. In the detailed analyses of M-174, we carried out comprehensive behavioral, pharmacological, histological, biochemical, hematological, blood biochemical tests and evaluated the mutant as an AD/HD model. Additionally, we identified a mutation in M-1736 and carried out pharmacological and histological analyses.

Research Products

• [Journal Article] Phenotypic characterization of a new Grin1 mutant mouse generated by ENU mutagenesis. (2010)
  • Author(s): Furuse T, Wada Y, Hattori K, Yamada I, Kushida T, Shibukawa Y, Masuya H, Kaneda H, Miura I, Seno N, Kanda T, Hirose R, Toki S, Nakanishi K, Kobayashi K, Sezutsu H, Gondo Y, Noda T, Yuasa S, Wakana S.
  • Journal Title: Eur J Neurosci. 31(7) Pages: 1281-1291
  • Peer Reviewed
• [Journal Article] Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center. (2009)
  • Author(s): Wakana S., Suzuki T., Furuse T., Kobayashi K., Miura I., Kaneda H., Yamada I., Motegi H., Toki H., Inoue M., Minowa O., Noda T., Waki K., Tanaka N., Masuya H., Obata Y.
  • Journal Title: Exp Anim. 58(5) Pages: 443-450
  • Peer Reviewed
• [Presentation] Phenotypic analyses of an ENU mutant mouse which exhibits hyper-locomotor activit (2010)
  • Author(s): 古瀬民生, 他
  • Organizer: BMB2010
  • Place of Presentation: 神戸コンベンションセンター・神戸市
  • Year and Date: 2010-12-07
• [Presentation] ENUミュータジェネシスによる注意欠如・多動性障害(AD/HD)モデルマウスの開発 (2010)
  • Author(s): 古瀬民生、若菜茂晴
  • Organizer: 第3回疾患モデルシンポジウム
  • Place of Presentation: 中央大学駿河台記念館・東京
  • Year and Date: 2010-11-18
• [Presentation] ENUミュータジェネシスにより作出された高活動変異マウスの表現型解析 (2010)
  • Author(s): 古瀬民生, 他
  • Organizer: NEURO2010
  • Place of Presentation: 神戸コンベンションセンター・神戸市
  • Year and Date: 2010-09-02
• [Presentation] マウス表現型解析基盤の標準化にもとづく行動解析-理研ENUミュータジェネシスプロジェクトからマウスクリニックへ (2009)
  • Author(s): 古瀬民生、若菜茂晴
  • Organizer: 社会性認識(学際脳科学シンポジウム)
  • Place of Presentation: 金沢大学・金沢市
  • Year and Date: 2009-11-05
• [Presentation] Comprehensive phenotype analyses of ENU induced mutant mouse that carries a missense mutation in Grin1 gene. (2009)
  • Author(s): Furuse T., et al.
  • Organizer: 39th Annual Meeting of the Society for Neuroscience (Neuroscience 2009).
  • Place of Presentation: Chicago, IL, USA.
  • Year and Date: 2009-10-20
• [Remarks] ホームページ等
  • URL: http://www.brc.riken.go.jp/lab/jmc/mouse_clinic/m-strain_jp.html
• [Remarks]
  • URL: http://www.brc.riken.jp/lab/animal/mailnews/nm201006_01.html