2010 Fiscal Year Final Research Report
A systematic search for potential substrates of deubiquitinase with quantitative proteomics
| Project/Area Number |
21710200
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OTA Kazuhisa The University of Tokyo, 大学院・理学系研究科, 助教 (00322727)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 翻訳後修飾 / ユビキチン |
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| Research Abstract |
Combining our originally poly-ubiquitinated protein enrichment method and high throughput LC-MS/MS analysis, we tried to identify specific substrates for all USP type deubiquitinases coded in the genome of Saccharomyces cerevisiae. Finally, we identified 94 potential substrate-enzyme relationships and, for some of these, made validation with molecular biological methods.
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