Crosstalk between the local temperature change inside single living cells and the cellular functions

2010 Fiscal Year Final Research Report

• Project/Area Number: 21740316
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics/Chemical physics
• Research Institution: Waseda University
• Principal Investigator: SUZUKI Madoka Waseda University, 総合研究機構, 講師 (40350475)
• Project Period (FY): 2009 – 2010
• Keywords: 生物物理

Research Abstract

The purpose of this study is to clarify the relationship between the temperature and the functions of the brain and the heart in which the [Ca^<2+>] strongly coupled with their function in the microscopic view point. We first focused on clarifying the thermal properties of single living cells. Then the effect of the local temperature gradient was studied with the use of dividing single HeLa cells. Furthermore, the response of the single motile cells to the local temperature gradient and the heat pulse was analyzed. In the meantime, the research was expanded to the studies to elucidate the mechanism of the oscillatory movement of the cardiac cell.

Research Products

• [Journal Article] Molecular motors as an auto-oscillator. (2010)
  • Author(s): Ishiwata, S., Shimamoto, Y., Suzuki, M.
  • Journal Title: HFSP J. 4(3) Pages: 100-104
  • Peer Reviewed
• [Presentation] Microscopic heat pulse induced calcium dynamics in a migratory cell. (2011)
  • Author(s): Itoh, H., Suzuki, M., Oyama, K., Motoyoshi, M., Ishiwata, S.
  • Organizer: 米国生物物理学会第55回年会(Biophysical Society the 55th Annual Meeting)
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 20110305-20110309
• [Presentation] Rearrangement of mitotic spindle in HeLa cell under steep temperature gradient. (2011)
  • Author(s): Motoyoshi, M., Suzuki, M., Oyama, K., Itabashi, T., Ishiwata, S.
  • Organizer: 米国生物物理学会第55回年会(Biophysical Society the 55th Annual Meeting)
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 20110305-20110309
• [Presentation] レーザー誘起気泡で生じる温度変化の時空間分布 (2011)
  • Author(s): 鈴木団
  • Organizer: 第3回FBT(生物学・化学・情報科学融合のための戦略的研究基盤形成支援事業)シンポジウム
  • Place of Presentation: 早稲田大学、東京
  • Year and Date: 2011-01-22
• [Presentation] Microscopic measurement of periodic rigor cross-bridge formation and their unbinding force in rabbit skeletal myofibril. (2010)
  • Author(s): Suzuki, M., Ishiwata, S.
  • Organizer: The 48th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sendai
  • Year and Date: 20100920-20100922
• [Presentation] High-speed imaging of heat transfer in single living cells. (2010)
  • Author(s): Oyama, K., Suzuki, M., Tseeb, V., Seto, K., Motoyoshi, M., Itoh, H., Iwai, K., Ishiwata, S.
  • Organizer: The 48th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sendai
  • Year and Date: 20100920-20100922
• [Presentation] Ca^<2+> response to microscopic heat pulses in a migrant cell. (2010)
  • Author(s): Itoh, H., Suzuki, M., Oyama, K., Motoyoshi, M., Seto, Y., Ishiwata, S.
  • Organizer: The 48th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sendai
  • Year and Date: 20100920-20100922
• [Presentation] Rotation of mitotic spindle in HeLa cell under steep temperature gradient. (2010)
  • Author(s): Motoyoshi, M., Suzui, M., Oyama, K., Itabashi, T., Ishiwata, S.
  • Organizer: The 48th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sendai
  • Year and Date: 20100920-20100922
• [Presentation] Mechanobiology in Muscle Contraction. (2010)
  • Author(s): Ishiwata, S., Shimamoto, Y., Ohtaki, M., Sato, K., Suzuki, M.
  • Organizer: The 48th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sendai
  • Year and Date: 20100920-20100922
• [Presentation] Microscopic measurement of periodic cross-bridge formation in skeletal myofibril. (2010)
  • Author(s): Suzuki, M., Ishiwata, S.
  • Organizer: 米国生物物理学会第54回年会Biophysical Society 54th Annual Meeting, San Francisco, CA
  • Place of Presentation: Ishiwata
  • Year and Date: 20100200
• [Presentation] Self-organization in Biomotile Systems-Molecular motors, Auto-oscillation (SPOC) in muscle and Meiotic spindle- (2010)
  • Author(s): Y.Oguchi, S.V.Mikhailenko, M.Suzuki, K.Sato, M.Ohtaki, Y.Shimamoto, K.Suzuki, J.Takagi, T.Itabashi
  • Organizer: 1st POSTECH Workshop on Physics of Self-Organization in Bio/Nano-Systems, Asia Pacific Center for Theoretical Physics (APCTP), Pohang, S.Korea
  • Year and Date: 20100127-20100129
• [Presentation] 拍動に伴う心筋細胞の熱産生イメージング (2010)
  • Author(s): 瀬戸祐介、大山廣太郎、新谷正嶺、福田紀男、鈴木団、石渡信一
  • Organizer: 2010年生体運動研究合同班会議プログラム
  • Place of Presentation: 中央大学、東京
  • Year and Date: 20100109-20100111
• [Presentation] Investigation of the role of micro-level physical parameters in biological systems. (2010)
  • Author(s): Suzuki, M.
  • Organizer: The 1st Anniversary Symposium of Waseda Bioscience Research Institute in Singapore (WABIOS)
  • Place of Presentation: Singapore
  • Year and Date: 2010-09-15
• [Presentation] Fluorescence imaging of a heat transfer in single HeLa cells. (2010)
  • Author(s): Oyama, K., Suzuki, M., Tseeb, V., Seto, Y., Motoyoshi, M., Itoh, H., Iwai, K., Ishiwata, S.
  • Organizer: The 1st Anniversary Symposium of Waseda Bioscience Research Institute in Singapore (WABIOS)
  • Place of Presentation: Singapore
  • Year and Date: 2010-09-15
• [Presentation] Development of microscopic imaging techniques and study into cell structure, physical properties and function. (2010)
  • Author(s): Suzuki, M., Oyama, K., Seto, Y., Ishiwata, S.
  • Organizer: 第2回FBT(生物学・化学・情報科学融合のための戦略的研究基盤形成支援事業)、PBL(大学院教育改革支援プログラム)ジョイントシンポジウム
  • Place of Presentation: 早稲田大学、東京
  • Year and Date: 2010-01-23
• [Presentation] Chemo-mechanical Feedback Loop in Biomotile Systems, International Conference on Physics Biology Interface (2009)
  • Author(s): S.Ishiwata, Y.Oguchi, S.V.Mikhailenko, M.Suzuki, K.Sato, M.Ohtaki, Y.Shimamoto, K.Suzuki, J.Takagi, T.Itabashi
  • Organizer: Saha Institute of Nuclear Physics
  • Place of Presentation: Kolkata, India
  • Year and Date: 20091213-20091216
• [Presentation] Direct observation of interaction between single actin filament and myosin in skeletal myofibril. (2009)
  • Author(s): Suzuki, M., Ishiwata, S.
  • Organizer: The 3rd Mechanobiology Workshop, National University of Singapore
  • Place of Presentation: Singapore
  • Year and Date: 20091103-20091105
• [Presentation] 分裂期のHeLa細胞への局所温度勾配の効果Effect of local temperature gradient on HeLa cells in mitotic phase. (2009)
  • Author(s): Motoyoshi, M., Suzuki, M., Oyama, K., Itabashi, T., Ishiwata, S.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
  • Year and Date: 20091030-20091101
• [Presentation] 肥満細胞の脱顆粒メカニズム:アクチン骨格再構成での活性化タンパク質Cの役割Mechanism of mast cell degranulation : Roles of activated protein C in the rearrangement of actin cytoskeleton. (2009)
  • Author(s): Ono, F., Suzuki, M., Oyama, K., Strukova, S., Ishiwata, S.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
  • Year and Date: 20091030-20091101
• [Presentation] 細胞内の局所的な温度変化の測定とその解析Measurement and analysis of local temperature change inside living cells. (2009)
  • Author(s): Oyama, K., Suzuki, M., Tseeb, V., Ono, F., Seto, Y., Motoyoshi, M., Iwai, K., Ishiwata, S.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
  • Year and Date: 20091030-20091101