2010 Fiscal Year Final Research Report
Development of enzymatic oxidation system in liposomes using gas-liquid flow for controlling the enzyme activity
| Project/Area Number |
21760642
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Yamaguchi University |
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Principal Investigator |
YOSHIMOTO Makoto Yamaguchi University, 大学院・医学系研究科, 准教授 (80322246)
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| Project Period (FY) |
2009 – 2010
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| Keywords | バイオリアクター / 酵素反応 / 生体機能利用 / ナノバイオ / 化学工学 |
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| Research Abstract |
The permeabilization of phospholipid bilayer membrane of liposomes to a low molecular mass compound was induced by the liquid shear stress at high temperatures. The catalase-bound liposome encapsulating glucose oxidase was prepared and its permeabilization with respect to glucose and oxygen was induced by the liquid shear stress generated by the gas-liquid flow in an external loop airlift bubble column. The liposomal enzyme system prepared functioned as a reactive and stable catalyst for the oxidation of glucose with decomposition of hydrogen peroxide produced in the bubble column.
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