2010 Fiscal Year Final Research Report
Establishment of chicken pluripotent stem cells with germ line competency
| Project/Area Number |
21760643
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyushu University |
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Principal Investigator |
KAWABE Yoshinori Kyushu University, 工学研究院, 助教 (30448401)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 多能性幹細胞 / トランスジェニック鳥類 / キメラニワトリ / フィーダー細胞 |
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| Research Abstract |
To establish chicken induced pluripotent stem cells, some chicken homologues to regulate pluripotency could be obtained. Retroviral vectors encoding their homologues were produced and infected into STO cells. Chicken blastodermal cells were cultured on genetically modified STO cells and the undifferentiated state was investigated. Chicken embryonic fibroblasts transducted by some retroviral vectors were evaluated by RT-PCR and AP staining. These results suggest that further improvement to induce chicken pluripotent stem cells is required.
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