2010 Fiscal Year Final Research Report
Construction of system and analysis of related factors for de novo centromere formation in fission yeast
| Project/Area Number |
21770009
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kurume University |
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Principal Investigator |
SATO Hiroshi Kurume University, 分子生命科学研究所, 助教 (00421313)
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| Project Period (FY) |
2009 – 2010
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| Keywords | セントロメア / 染色体分 / 裂酵母 |
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| Research Abstract |
Artificial fused chromosome containing two centromeres were converted into a stable monocentric chromosome by functionally inactivating one centromere in fission yeast. We developed a method to generate de novo centromere from the fused chromosome on which the active centromere was deleted by site-directed recombination and only inactive centromere was contained. The analysis using this system in mutant strains indicated that the heterochromatin and deacetylation of histone prevent revival of the inactivated centromere.
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