2010 Fiscal Year Final Research Report
The determination of disulfide connectivity and conformation by the SAIL method
| Project/Area Number |
21770110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKEDA Mitsuhiro Nagoya University, 理学研究科, 研究員 (90508558)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 蛋白質 / NMR |
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| Research Abstract |
The determination of disulfide connectivity and conformation in a protein is essential for understanding the structural stability of the protein. However, no method for investigating them has been established. Here, a method for observing cross-bridge NOEs involving two b-protons of different cysteine residues has been developed. In a conventional uniformly isotope-labeled protein, the observation of such a NOE is difficult due to signal overlap and spin diffusion effect. In our approach, a protein whose protons are exclusively deuterated except for the two Cys's β-protons is employed, such that the connectivity and proton-proton distance can readily be determined.
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