2010 Fiscal Year Final Research Report
Structural basis of the complex proteins involved in the iron-sulfur cluster biogenesis
| Project/Area Number |
21770112
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
WADA Kei Osaka University, 大学院・理学研究科, 助教 (80379304)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 鉄硫黄クラスター / X線結晶構造解析 / ATP分解酵素 / SUFマシナリー / ISCマシナリー |
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| Research Abstract |
In this study, the quaternary structure of the SufC_2-SufD_2 complex involved in Fe-S cluster biogenesis in which SufC is bound to the C-terminal domain of SufD was determined. Comparison with the monomeric structure of SufC revealed conformational change of the active-site residues : SufC becomes competent for ATP binding and hydrolysis upon association with SufD. These findings, together with biochemical characterization of the SufB-SufC-SufD complex, have led us to propose a model for the Fe-S cluster biosynthesis in the complex.
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[Journal Article] Molecular Dynamism of Fe-S Cluster Biosynthesis Implicated by the Structure of the SufC_2-SufD_2 Complex.2009
Author(s)
Wada, K., Sumi, N., Nagai, R., Iwasaki, K., Sato, T., Suzuki, K., Hasegawa, Y., Kitaoka, S., Minami, Y., Outten, W., F., Takahashi, Y., Fukuyama, K
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Journal Title
K.J.Mol.Biol. 387
Pages: 245-258
Peer Reviewed
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[Presentation] Crystal Structure of the SufC2-SufD2 Complex : Implications for Dynamic Rearrangement During Fe-S cluster Biosynthesis.2009
Author(s)
Wada, K., Sumi, N., Nagai, R., Iwasaki, K., Sato, T. Suzuki, K., Nagasaka, Y., Minami., Y., Outten, F.W., Takahashi, Y., Fukuyama., K.
Organizer
Iron-Sulfur Cluster Biogenesis and Regulation Conference
Place of Presentation
Geogia, USA
Year and Date
2009-09-01
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