2010 Fiscal Year Final Research Report

Identification of post-translational modification in nuclear proteins during neural stem cell fate specification by proteomic analysis

Research Project

Project/Area Number	21770119
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Structural biochemistry
Research Institution	Kumamoto University
Principal Investigator	NIIMORI Kanako Kumamoto University, 大学院・生命科学研究部, 助教 (30457600)
Project Period (FY)	2009 – 2010
Keywords	神経幹細胞 / プロテオミクス / 翻訳後修飾 / リン酸化 / 2D-DIGE / 分化制御 / 転写調節因子 / 質量分析
Research Abstract	It is not yet completely understood how neural stem cell fate is specified, in spite of extensive investigations by, for instance, gene expression profiling. To tackle this important question, we have focused on the changes in post-translational modification of nuclear proteins such as transcription factors, coactivators/corepressors, and chromatin modifiers in neural stem cells (NSCs) in response to fibroblast growth factor 2 (FGF2), a representative factor to maintain them. NSCs were stimulated by FGF2, and its lysates were examined by proteomic analysis. The result revealed 80 up- or down-regulated protein spots upon FGF2 stimulation out of 4095 spots with statistically significant differences. Among them, we focused on 24 spots differentially phosphorylated in response to FGF2, and identified all of them by nanoLC-QqTOF MS analysis. These proteins contained factors related to nuclear dynamics including nuclear translocation and chromatin modifications.