2010 Fiscal Year Final Research Report
Physiological roles of enzymatic activity and posttranslational modifications of circadian regulators
| Project/Area Number |
21770138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HIRAYAMA Jun Tokyo Medical and Dental University, 難治疾患研究所, 准教授 (90510363)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 概日リズム / 基本生理機能 / 転写 / 分子時計 / 翻訳後修飾 |
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| Research Abstract |
Accumulating evidence suggests that the death domain-associated protein DAXX has a variety of functions in regulating apoptotic pathways. In this study, we have identified BMAL1, an essential circadian regulator, as a novel DAXX-interacting protein. The temporal association of circadian clock components is required for establishing and maintaining circadian rhythms. Notably, the interaction between endogenous DAXX and BMAL1 showed a circadian rhythmicity. Small interfering RNA (siRNA)-mediated silencing of endogenous DAXX expression and genetic ablation of the Daxx gene affected the amplitude of circadian gene expression in mammalian cells. In addition, DAXX enhanced the transcriptional capacity of the BMAL1:CLOCK heterodimer, a vital transcriptional activator in the circadian system. Our results reveal a novel function for DAXX as a modulator of circadian clock regulation.
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Research Products
(14 results)
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[Journal Article] p38 Mitogen-Activated Protein Kinase Controls a Switch between Cardiomyocyte and Neuronal Commitment of Murine Embryonic Stem Cells by Activating MEF2C-dependent BMP2 Transcription.2010
Author(s)
Wu J, Kubota J, Hirayama J, Nagai Y, Nishina S, Yokoi T, Asaoka Y, Seo J, Shimizu N, Kajiho H, Watanabe T, Azuma N, Katada T, Nishina H.
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Journal Title
Stem Cells and Development 19
Pages: 1723-1734
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[Journal Article] Negative regulation of wnt11 by JNK signaling is required for convergent extension during vertebrate gastrulation.2010
Author(s)
Seo J, Asaoka Y, Nagai Y, Hirayama J, Yamasaki T, Namae M, Ohata S, Shimizu N, Negishi T, Kitagawa D, Kondoh H, Furutani-Seiki M, Penninger JM, Katada T, Nishina H.
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Journal Title
J.Cell.Biochem. 110
Pages: 1022-1037
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[Journal Article] Filamin Associates with Stress Signaling Kinases MKK7 and MKK4 and Regulates JNK Activation.2010
Author(s)
Nakagawa K, Sugahara M, Yamasaki T, Kajiho H, Takahashi S, Hirayama J, Minami Y, Ohta Y, Watanabe T, Hata Y, Katada T, Nishina H.
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Journal Title
Biochem.J. 427
Pages: 237-245
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