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2011 Fiscal Year Final Research Report

Structural change and function of ClpB chaperone disaggregase

Research Project

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Project/Area Number 21770151
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionKonan University

Principal Investigator

WATANABE Yo-hei  甲南大学, 理工学部, 講師 (40411839)

Project Period (FY) 2009 – 2011
Keywords酵素の触媒機構
Research Abstract

ClpB can rescue the aggregated proteins(disaggregation). When the motion of N domain was restricted by the disulfide bond, the disaggregation activity of ClpB reduced. We found that the cause is in the threading-through process following the substrate-binding process. Moreover, we made two ClpB mutants in which two or six adjacent subunits of ClpB hexamer were cross-linked by the disulfide bond(s). Using these mutants, we found that the efficient disaggregation by ClpB required the moderate dissociation/association of the hexamer.

  • Research Products

    (12 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results)

  • [Journal Article] Orientation of the amino-terminal domain of ClpB affects the disaggregation of the protein2012

    • Author(s)
      Sayaka Mizuno, Yosuke Nakazaki, Masasuke Yoshida, Yo-hei Watanabe
    • Journal Title

      FEBS J.

      Volume: 279 Pages: 1474-1484

    • DOI

      DOI:10.1111/j.1742-4658.2012.08540.x

    • Peer Reviewed
  • [Journal Article] Roles of conserved arginines in ATP-binding domains of AAA+chaperone ClpB from Thermus thermophilus2011

    • Author(s)
      Takashi Yamasaki, Yosuke Nakazaki, Masasuke Yoshida, Yo-hei Watanabe
    • Journal Title

      FEBS J.

      Volume: 278 Pages: 2395-2403

    • DOI

      DOI:10.1111/j.1742-4658.2011.08167.x

    • Peer Reviewed
  • [Journal Article] Temperature-dependent regulation of Thermus thermophilus DnaK/DnaJ chaperone by DafA protein2009

    • Author(s)
      Tadashi Mizutani, Shohei Nemoto, Masasuke Yoshida, Yo-hei Watanabe
    • Journal Title

      Genes to Cells

      Volume: 14 Pages: 1405-1413

    • DOI

      DOI:10.1111/j.1365-2443.2009.01357.x

    • Peer Reviewed
  • [Journal Article] Stability of the two wings of the coiled-coil domain of ClpB chaperone is Critical for its disaggregation activity2009

    • Author(s)
      Yo-hei Watanabe, Yosuke Nakazaki, Ryoji Suno, Masasuke Yoshida
    • Journal Title

      Biochem. J.

      Volume: 421 Pages: 71-77

    • DOI

      DOI:10.1042/BJ20082238

    • Peer Reviewed
  • [Presentation] 分子シャペロンClpBのサブユニット間の協同性2011

    • Author(s)
      山崎孝史、中村俊樹、吉田賢右、渡辺洋平
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-23
  • [Presentation] 分子シャペロンClpBの6量体構造の安定性とシャペロン活性との関係2010

    • Author(s)
      中村俊樹、山崎孝史、本多大輔、吉田賢右、渡辺洋平
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
  • [Presentation] 分子シャペロンClpB変異体の基質糸通し速度の測定2010

    • Author(s)
      中崎洋介、水野さやか、吉田賢右、渡辺洋平
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
  • [Presentation] 分子シャペロンClpBのアルギニンフィンガーの役割2010

    • Author(s)
      山崎孝史、中村俊樹、吉田賢右、渡辺洋平
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
  • [Presentation] Roles of the motion of the N-terminal domain of ClpB chaperone2010

    • Author(s)
      Yosuke Nakazaki, Sayaka Mizuno, Masasuke Yoshida, Yo-hei Watanabe
    • Organizer
      The 3rd International Symposium on Protein Community
    • Place of Presentation
      奈良
    • Year and Date
      2010-09-14
  • [Presentation] 分子シャペロンClpBにおけるミドルドメインWing-2の保存荷電残基の重要性2009

    • Author(s)
      渡邊久美子、中村俊樹、中崎洋介、水野さやか、吉田賢右、渡辺洋平
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
  • [Presentation] ClpB変異体の基質糸通し活性の解析2009

    • Author(s)
      中崎洋介、水野さやか、渡邊久美子、中村俊樹、吉田賢右、渡辺洋平
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
  • [Presentation] サブユニット間ジスルフィド結合による分子シャペロンClpBの6量体構造の固定化2009

    • Author(s)
      中村俊樹、中崎洋介、渡辺洋平
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22

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Published: 2013-07-31  

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