Molecular genetic analysis of cadmium accumulation in rice

2011 Fiscal Year Final Research Report

• Project/Area Number: 21780007
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Akita Prefectural University
• Principal Investigator: NAGASAWA Namiko 秋田県立大学, 生物資源科学部・生物生産科学科, 助教 (00535289)
• Project Period (FY): 2009 – 2011
• Keywords: 植物育種・遺伝 / イネ / 突然変異体 / カドミウム

Research Abstract

This research revealed how cadmium is transported from root to shoot in cadmium hyper-accumulating rice cultivar, Cho-Ko-Koku. The main gene in the mechanism was OsHMA3. OsHMA2, a homolog of OsHMA3, also functions in the pathway of cadmium transport in rice. In addition, the genetic screen was conducted in this research and several mutants were isolated.

Research Products

• [Journal Article] Mutations in rice(Oryza sativa) heavy metal ATPase 2(OsHMA2) restrict the translocation of Zn and Cd (2012)
  • Author(s): Satoh-Nagasawa N., Mori M., Nakazawa N., Kawamoto T., Nagato Y., Sakurai K., Takahashi H., Watanabe A., Akagi H.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 213-224
  • Peer Reviewed
• [Journal Article] OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles (2011)
  • Author(s): Miyadate, H., Adachi, S., Hiraizumi, A., Tezuka, K., Nakazawa, N., Kawamoto, T., Katou, K., Kodama, I., Sakurai, K., Takahashi, H., Satoh-Nagasawa, N., Watanabe, A., Fujimura, T., Akagi, H.
  • Journal Title: New Phytologist Volume: 189 Pages: 190-199
  • Peer Reviewed
• [Journal Article] A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku (2010)
  • Author(s): Tezuka, K., Miyadate, H., Katou, K., Kodama, I., Matsumoto, S., Kawamoto, T., Masaki, S., Satoh, H., Yamaguchi, M., Sakurai, K., Takahashi, H., Satoh-Nagasawa, N., Watanabe, A., Fujimura, T., Akagi, H.
  • Journal Title: Theoretical and Applied Genetics Volume: 120 Pages: 1175-1182
  • DOI: DOI:10.1007/s00122-009-1244-6.
  • Peer Reviewed
• [Presentation] OsHMA2 mutants are blocked in Zn and Cd transport (2012)
  • Author(s): Satoh-Nagasawa N., Mori M., Nakazawa N., Kawamoto T., Nagato Y., Sakurai K., Takahashi H., Watanabe A., Akagi H.
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] レトロトランスポゾンTos17挿入系統を用いたイネOsHMA2遺伝子の機能解析 (2011)
  • Author(s): 佐藤(永澤)奈美子・森美果子・川本朋彦・長戸康郎・櫻井健二・高橋秀和・渡辺明夫・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-23
• [Presentation] イネのカドミウム移行におけるP1B-type heavy-metal ATPaseの機能の解析 (2010)
  • Author(s): 森美果子・宮舘秀典・川本朋彦・櫻井健二・高橋秀和・渡辺明夫・佐藤(永澤)奈美子・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24
• [Presentation] カドミウム高蓄積性イネ'長香穀'における地上部へのカドミウム移行機構 (2010)
  • Author(s): 平泉彩・安達早希・宮舘秀典・手塚耕一・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-03-27
• [Presentation] イネのカドミウム移行を高める遺伝子qCdT7の単離 (2009)
  • Author(s): 宮舘秀典・手塚耕一・安達早紀・平泉彩・佐藤奈美子・渡辺昭夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-25
• [Presentation] カドミウム高吸収性イネ'長香穀'のカドミウム蓄積性に関与するQTL (2009)
  • Author(s): 手塚耕一・宮舘秀典・佐藤奈美子・渡辺明夫・櫻井健二・高橋秀和・川本朋彦・加藤和直・小玉郁子・眞崎聡・佐藤秀樹・山口誠之・赤木宏守
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-25
• [Remarks]
  • URL: http://www.akita-pu.ac.jp/stic/souran/scholar/detail.php?id=224
• [Remarks]
  • URL: http://www.akita-pu.ac.jp/stic/souran/study/detail.php?id=233