2010 Fiscal Year Final Research Report
Optimization of technologies for improving microbial metabolisms using antisense RNA expression system
| Project/Area Number |
21780071
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
HIRASAWA Takashi Osaka University, 大学院・情報科学研究科, 助教 (20407125)
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| Project Period (FY) |
2009 – 2010
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| Keywords | アンチセンスRNA / コリネ型細菌 / グルタミン酸 / 2-オキソグルタル酸デヒドロゲナーゼ複合体 / odhA |
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| Research Abstract |
In this study, effectiveness of antisense RNA expression system was examined using antisense RNA expression system for odhA mRNA of a coryneform bacterium Corynebacterium glutamicum. Moreover, effective design of antisense RNA for improving metabolisms and optimization of antisense RNA expression system were tried by constructing the antisense RNAs with various length and those based on the secondary structure of target mRNA.
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