Analysis of chromatin remodeling machinery required for the regulation of DNA replication

2012 Fiscal Year Final Research Report

• Project/Area Number: 21780086
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: OMA Yukako 東北大学, 大学院・農学研究科, 研究支援者 (20443997)
• Project Period (FY): 2010 – 2012
• Keywords: クロマチン / DNA複製 / アクチン関連タンパク質 / クロマチンリモデリング複合体

Research Abstract

To analyze the function of the INO80 chromatin remodeling complex in DNA replication and in the maintenance of genome stability, we established Degron yeast strains in which Ino80 and Arp8 are inducibly digested. We showed that the impairment of the INO80 complex caused genome instability. We also showed physiological and genetic interactions of the INO80 complex with DNA replication factors, chromatin proteins, and nuclear pore complex. Theseresults are important to reveal molecular mechanisms of the INO80 complex in DNAreplication and the maintenance of genome stability.

Research Products

• [Journal Article] 細胞核の構造とエピジェネティック制御 (2012)
  • Author(s): 尾間由佳子、原田昌彦
  • Journal Title: 化学と生物 Volume: 50 Pages: 262-268
  • URL: http://jglobal.jst.go.jp/public/20090422/201202237007967052
• [Journal Article] Actin-related proteins localized in the nucleus: from discovery to novel roles in nuclear organization (2011)
  • Author(s): Oma, Y., Harata, M
  • Journal Title: Nucleus Volume: 2 Pages: 38-46
  • DOI: doi:10.4161/nucl.2.1.14510.
  • Peer Reviewed
• [Journal Article] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot (2010)
  • Author(s): Sun, J., Oma, Y., Harata, M., Kono, K., Shima, H., Kinomura, A., Ikura, T., Mizutani, S., Kanaarm, R., Tashiro, S
  • Journal Title: PLoS One Volume: 5 Pages: e13554
  • DOI: doi:10.1371/journal.pone.0013554.
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp6 influences H2AZ-dependent and -independent gene expression and links ribosomal protein genes to nuclear pores (2010)
  • Author(s): Yoshida, T, Shimada, K., Oma, Y., Kalck, V., Akimura, K., Taddei, A., Iwahashi, H., Kugou, K., Ohta, K., Gasser, S.M., Harata, M
  • Journal Title: PLoS Genet Volume: 6 Pages: e1000910
  • DOI: doi:10.1371/journal.pgen.1000910.
  • Peer Reviewed
• [Presentation] The Function of SWR1- and INO80 Chromatin Remodeling Complexes in the Spatial Positioning of DNA Double-strand Break (2012)
  • Author(s): Yukako Oma
  • Organizer: The 35^<th> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-12-12
• [Presentation] Roles of actin-related proteins in chromatin function and nuclear organization (2012)
  • Author(s): Yukako Oma
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology, Workshop "Gene expression and Epigenetics"
  • Place of Presentation: Kobe
  • Year and Date: 2012-05-28
• [Presentation] DNA二本鎖切断(DSB)におけるクロマチンリモデリング複合体INO80複合体およびSWR1複合体の機能解析 (2011)
  • Author(s): 尾間由佳子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] ヒト染色体安定性維持におけるINO80クロマチンリモデリング複合体の機能解析 (2010)
  • Author(s): 尾間由佳子
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] ヒト染色体安定性維持におけるINO80クロマチンリモデリング複合体の機能解析 (2010)
  • Author(s): 尾間由佳子
  • Organizer: 日本農芸化学会東北支部・北海道支部合同支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-28