2010 Fiscal Year Final Research Report
Functional and structural analyses of the archaeal L-proline dehydrogenase complexes
| Project/Area Number |
21780099
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Saga University |
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Principal Investigator |
KAWAKAMI Ryushi Saga University, 総合分析実験センター, 助教 (90380120)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 酵素化学 / 構造生物学 |
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| Research Abstract |
Two different types of ProDHs (α_4β_4 and αβγδ type) were distributed in the hyperthermophilic archaea. Functional and structural characteristics of both ProDHs were studied. Structure determination at high resolution of α_4β_4-type ProDH which the ICS was not broken, and mutational analysis of intersubunit disulfide bond in α subunit of α_4β_4-type ProDH were performed. Screening of crystallization condition of αβγδ type ProDH was also performed by preparing the ISC mutant enzymes. And the enzymatic properties of β subunits of both types of ProDHs were analyzed.
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Research Products
(5 results)
