Linkage mapping and eQTL analysis of candidate resistant gene for pine wilt disease caused by wood nematode

2010 Fiscal Year Final Research Report

• Project/Area Number: 21780157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: ISODA Keiya Forestry and Forest Products Research Institute, 林木育種センター関西育種場, 育種研究室長 (60391702)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Atsushi 独立行政法人森林総合研究所, 林木育種センター・育種第一課, 基盤技術研究室長 (10360471) ; HIRAO Tomonori 独立行政法人森林総合研究所, 森林バイオ研究センター, 研究員 (90457763)
• Project Period (FY): 2009 – 2010
• Keywords: 森林生産 / 育種 / 遺伝学 / 林木育種

Research Abstract

3'-RACE was conducted using previously reported EST information of candidate resistant gene for pine wood nematode in order to determine 3'-end sequence of the ESTs. PCR primers were designed and single-genes were screened by sequencing haploid genome extracted from megagametophyto. Two primers were developed to amplify single-genes containing SNPs. Expression QTL analysis of pathogen related genes were conducted. EQTL of chitinase and PR-4 were detected in the same linkage group of phenotypic resistant trait, suggesting similar regulation factor was related to these genes and resistance