2011 Fiscal Year Final Research Report

Regulation of autophagy by herpesvirus protein : the involvement in neurodegenerative disorder

Research Project

Project/Area Number	21780260
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	Hokkaido University
Principal Investigator	TOMIOKA Yukiko 北海道大学, 遺伝子病制御研究所, 助教 (50374674)
Project Period (FY)	2009 – 2011
Keywords	病態 / 疾患モデル / 発生工学 / ウイルス / 脳・神経疾患 / オートファジー
Research Abstract	To verify that protein of herpes virus induce neurodegeneration via inhibition of cellular autophagy, the transgenic mice expressing IE180, immediate early protein of suid herpesvirus 1, were examined. P62 protein, which were degraded via selectively autophagic pathyway, and abnormal lysosome were aggregated in neuronal cells of IE180 transgenic mice. Expression of IE180 also induced accumulation of p62 in vitro. Double transgenic mice crossed IE180 transgenic mice with GFP-LC3 transgenic mice, expressing the green fluorescent protein fused to the autophagic marker LC3, were generated and examined. It seemed that IE180 transiently induced activation of autophagy, but then prevent normal autophagy and result in cell degeneration