Functional Analysis of Nucleoplasmin in Mammalian Oocyte Nucleolus

2010 Fiscal Year Final Research Report

• Project/Area Number: 21780270
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OGUSHI Sugako The Institute of Physical and Chemical Research, 生殖系列研究チーム, 基礎科学特別研究員 (50437505)
• Project Period (FY): 2009 – 2010
• Keywords: 卵母細胞 / 核小体 / 初期胚発生 / ヌクレオプラスミン2(Npm2)

Research Abstract

In mammals, the oocyte nucleolus is required for early embryonic development, but the function of oocyte nucleolus is elusive. Detailed analysis revealed that the zygotes lacking nucleolus showed the delay of sperm chromatin decondensation, constitutive heterochromatin disorganization during interphase, and the defect in first mitotic progression. These results indicate that the oocyte nucleolus is involved in the functional chromatin organization in zygotes. Moreover, we identified the proteins interacted with nucleoplasmin2 (Npm2) in the oocyte nucleolus. The function of these proteins is under analysis.

Research Products

• [Journal Article] Nucleoli from Growing Oocytes Support the Development of Enucleolated Full-grown Oocytes in the Pig. (2010)
  • Author(s): Kyogoku, H., Ogushi, S., Miyano, T
  • Journal Title: Molecular Reproduction and Development,Selected for Cover. 77 Pages: 167-173
  • Peer Reviewed
• [Journal Article] The Nucleolus in the Mouse Oocyte Is Required for the Early Step of Both Female and Male Pronucleus Organization. (2010)
  • Author(s): Ogushi S., Saitou M
  • Journal Title: Journal of Reproduction and Development,Selected for Cover 56 Pages: 495-501
  • Peer Reviewed
• [Journal Article] Involvement of Mouse Nucleoplasmin 2 in the Decondensation of Sperm Chromatin after Fertilization.
  • Author(s): Inoue A., Ogushi S., Saitou M., Suzuki MG., Aoki F
  • Journal Title: Biology of Reproduction (In press.)
  • Peer Reviewed
• [Presentation] Oocyte Nucleolus Requirement for the Higher Chromatin Organization in Zygotes. (2011)
  • Author(s): Ogushi S., Yamanaka K., Itoi F., Yamagata K., Wakayama T., Saitou M
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities
  • Place of Presentation: Awaji, Japan.
  • Year and Date: 20110100
• [Presentation] Functional Analysis of Maternal Nucleolus in Mouse Oocytes. (2010)
  • Author(s): Ogushi S., Kato Y., Nakamura A., Saitou M
  • Organizer: Czech-Japan Joint Symposium for Animal Reproduction, FROM GAMETES TO STEM CELLS, Liblice
  • Place of Presentation: Czech Republic.
  • Year and Date: 20100900
• [Presentation] The Maternal Nucleolus Function in Early Embryonic Development. (2010)
  • Author(s): Ogushi S., Kato Y., Nakamura A., Saitou M
  • Organizer: 43rd Annual Meeting for the Japanese Society of Developmental Biolosists
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 20100600
• [Presentation] The oocyte nucleolus roles during early embryonic development in mouse. (2009)
  • Author(s): Ogushi, S
  • Organizer: 2009 Japan-Taiwan Joint Symposium on Cell Signaling and Gene Regulation
  • Place of Presentation: Kobe, Japan.
  • Year and Date: 20091100
• [Presentation] The nucleolus in the mouse oocyte is required for the early step of pronucleus formation (2009)
  • Author(s): Ogushi, S
  • Organizer: 18th Lake Shirakaba Conference
  • Place of Presentation: Vedbaek, Denmark.
  • Year and Date: 20090600
• [Remarks] ホームページ等該当なし