2012 Fiscal Year Final Research Report
Development of oral inactivated recombinant Salmonella vaccine using E.coli cell surface display technique
Project/Area Number |
21780290
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Clinical veterinary science
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Research Institution | Osaka Prefecture University |
Principal Investigator |
TANI Hiroyuki 大阪府立大学, 生命環境科学研究科, 准教授 (00305658)
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Project Period (FY) |
2009 – 2012
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Keywords | サルモネラ / 不活化経口ワクチン / 大腸菌表面発現 |
Research Abstract |
In order to develop oral inactivated Salmonella Enteritidisvaccine, tne E. coli surface display plasmid vector was newly constructed in this study. The N-terminal domain of MisL, which is classified as Salmonella type V secretion system, was employed an anchor protein for surface display of recombinant protein. The fusion proteins of fully functional cholera toxin B subunit (CTB), which is one of the suitable candidates for mucosal adjuvant, and Salmonella Enteritidis vaccine candidate proteins were able to be displayed on the surface of E. coli. The chickens were immunized orally with the inactivated E. coli expressed fusion proteins. The CTB-specific IgA titers in intestinal mucus were enhanced. Our findings obtained in this study can be applied to development of simple and safety oral SE vaccine.
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Research Products
(6 results)
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[Journal Article] Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan2011
Author(s)
Matsubayashi M, Carreno RA, Tani H, Yoshiuchi R, Kanai T, Kimata I, Uni S, Furuya M,Sasai K.
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Journal Title
Vet Parasitol
Volume: 176(2-3)
Pages: 270-4
Peer Reviewed
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