Analysis of phosphorylation of rice CPD photolyase

2011 Fiscal Year Final Research Report

• Project/Area Number: 21780294
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: Tohoku University
• Principal Investigator: TERANISHI Mika 東北大学, 大学院・生命科学研究科, 助教 (10333832)
• Project Period (FY): 2009 – 2011
• Keywords: 遺伝子資源 / 紫外線抵抗性植物

Research Abstract

Cyclobutane pyrimidine dimer (CPD)photolyase is a crucial factor for determining UVB sensitivity in rice. We found the possibility that the rice CPD photolyase was phosphorylated by cyclin-dependent kinase. Furthermore, it was thought that phosphorylation of CPD photolyase was not involved in its translocation to organelles in Arabidopsis. Phosphorylation of CPD photolyase was specifically detected in some of the poaceous species. In these species, phosphorylation of CPD photolyase might be involved in protein transport to specific organelles.

Research Products

• [Journal Article] Augmentation of CPD photolyase activity in japonica and indica rice increases their UVB resistance but still leaves the difference in their sensitivities (2012)
  • Author(s): Teranishi M, Taguchi T, Ono T, Hidema J
  • Journal Title: Photochem. Photobiol. Sci. Volume: 11 Pages: 812-820
  • Peer Reviewed
• [Journal Article] Eukaryotic class II CPD photolyase structure reveals a basis for improved UV-tolerance in plants. (2011)
  • Author(s): Hitomi K, Arvai AS, Yamamoto J, Hitomi C, Teranishi M, Hirouchi T, YamamotoK, Iwai S, Tainer JA, Hidema J, Getzoff ED
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 12060-12069
  • Peer Reviewed
• [Journal Article] Cyclobutane-pyrimidine dimer (CPD)photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA. (2011)
  • Author(s): Takahashi M, Teranishi M, Ishida H, Kawasaki J, Takeuchi A, Yamaya T, Watanabe M, Makino A, Hidema J
  • Journal Title: Plant J. Volume: 66 Pages: 433-442
  • Peer Reviewed
• [Presentation] Analysis of sub-cellular localization of triple targeting rice CPD photolyase (2012)
  • Author(s): Takahashi S, Teranishi M, Takahashi F, Takahashi M, Hidema J
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 奈良
  • Year and Date: 2012-03-21
• [Presentation] イネのtriple-targeting CPD光回復酵素のオルガネラ移行シグナル配列に関する解析 (2012)
  • Author(s): 高橋さやか、高橋正明、寺西美佳、日出間純
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] (6-4)光回復酵素がイネの紫外線抵抗性に与える影響 (2011)
  • Author(s): 寺西美佳、高橋祐子、宗村郁子、日出間純
  • Organizer: 第54回日本放射線影響学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-18
• [Presentation] Effect of phospho-rylation on function of rice CPD photolyase (2011)
  • Author(s): Teranishi M, Nakamura K, Furukawa H, Takahashi M, Hidema J
  • Organizer: Societas Physiologia Plantarum Scandinavica 2011
  • Place of Presentation: ノルウェー
  • Year and Date: 2011-08-23
• [Presentation] Phosphorylation of rice CPD photolyase (2011)
  • Author(s): Teranishi M, Nakamura K, Furukawa H, Takahashi M, Hidema J
  • Organizer: 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良
  • Year and Date: 2011-08-01
• [Presentation] イネCPD光回復酵素の細胞内局在について (2011)
  • Author(s): 高橋正明、寺西美佳、石田宏幸、高橋さやか、日出間純
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-20
• [Presentation] 植物CPD光回復酵素のリン酸化修飾に関する研究 (2011)
  • Author(s): 中村憲太郎、寺西美佳、日出間純
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-20
• [Presentation] イネCPD光回復酵素のリン酸化酵素に関する解析 (2011)
  • Author(s): 古川晴也、寺西美佳、日出間純
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-20
• [Presentation] 植物CPD光回復酵素のリン酸化修飾 (2010)
  • Author(s): 寺西美佳、高橋正明、中村憲太郎、古川晴也、日出間純
  • Organizer: 第53回日本放射線影響学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] イネCPD光回復酵素のリン酸化部位の同定 (2010)
  • Author(s): 寺西美佳、日出間純
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] CPD photolyase of rice is phosphorylated (2010)
  • Author(s): Teranishi M, Nakamura K, Takahashi M, Hidema J
  • Organizer: Plant DNA Repair and Recombination 2010
  • Place of Presentation: アメリカ
  • Year and Date: 2010-03-02