2011 Fiscal Year Final Research Report
Development of ultrasensitive bioluminescent immunoassay by using DNA amplification technique
| Project/Area Number |
21790042
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku Pharmaceutical University (2011)
Showa University (2009-2010) |
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Principal Investigator |
OHNO Ken-ichi 東北薬科大学, 薬学部, 講師 (20347272)
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| Project Period (FY) |
2009 – 2011
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| Keywords | イムノアッセイ / 遺伝子増幅法 / 生物発光検出 / 臨床検査 / 診断 / スクリーニング |
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| Research Abstract |
In this study, a highly sensitive immunoassay was developed by using DNA amplification technique as detection system. Under isothermal condition, LAMP(Loop-mediated isothermal Amplification) and RCA(Rolling Circle Amplification) reaction enable successive DNA amplification. In order to apply DNA amplification to the detection system on immunoassay, DNA-conjugated antigen was prepared for competitive immunoassay. As a result, physically and chemically stable DNA provided highly efficient and sensitive signal amplification based on genetic engineering.
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Research Products
(3 results)
