2011 Fiscal Year Final Research Report
a Novel Screening System for Potential Anticancer Drugs with Gene Amplification Method
Project/Area Number |
21790045
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Physical pharmacy
|
Research Institution | Tokyo University of Science |
Principal Investigator |
AKIRA Inoue 東京理科大学, 薬学部, 嘱託助教 (30516406)
|
Project Period (FY) |
2009 – 2011
|
Keywords | 遺伝子増幅 / コンビナトリアルケミストリー / 金コロイド粒子 / DNA結合分子 / スクリーニング |
Research Abstract |
DNA-binding molecules are potential anticancer drugs to regulate gene amplification or gene expressions. The accurate relative determination of DNA-binding affinities of chemical compounds is an important factor for drug discovery area. In this work, I have developed a novel system to evaluate relative DNA-binding affinities of chemical compounds with primer-dimer(PD) templates using a real-time PCR analyzer. The utility of PD templates allows us to be accurate and highly sensitive determination of DNA-binding affinities of chemical compounds without the amplification of non-specific PCR products. We also developed the colorimetric determination system of DNA-binding affinities of chemical compounds in combination with gold nanoparticle aggregation phenomena and PCR-based evaluation system described above.
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Research Products
(5 results)