2010 Fiscal Year Final Research Report
Essential role of HIF-1alpha in Mycobacterium tuberculosis-infected macrophage
| Project/Area Number |
21790130
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | Osaka City University |
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Principal Investigator |
OKA Mayuko Osaka City University, 大学院・医学研究科, 研究員 (40347498)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 低酸素 / 結核 / HIF-1alpha / マクロファージ / 肉芽腫 |
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| Research Abstract |
Hypoxia-inducible factor-1α (HIF-1α) protein expressed in mouse pulmonary granulomas 6 weeks after infected with Mycobacterium tuberculosis, and the expression colocalized with that of M. tuberculosis antigen. Thus we focused to a role of HIF-1α into macrophages accumulated in granulomas. Our resulted investigated that the survival of M. tuberculosis in bone marrow derived macrophages (BMDM) from HIF-1α deficient mouse (HIF-1α^<-/->) is enhanced, compared with that in wild type BMDM (WT), which are post-stimulated with interferon-γ (IFN-γ) after infected with M. tuberculosis H37Rv. When infected with bacillus, the mRNA levels of 6 kinds of glycolytic enzymes were elevated in WT compared with HIF-1α^<-/->. In this study, we suggested that an increase of bacillus is controlled by HIF-1α.
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[Journal Article] Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice.2010
Author(s)
Ozeki Y, Sugawara I, Udagawa T, Aoki T, Osada-Oka M, Tateishi Y, Hisaeda H, Nishiuchi Y, Harada N, Kobayashi K, Matsumoto S.
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Journal Title
Int.Immunol. 22(3)
Pages: 179-189
Peer Reviewed
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