2010 Fiscal Year Final Research Report
Study of the molecular compartment of primary cilia
| Project/Area Number |
21790187
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SHIBA Dai Kyoto Prefectural University of Medicine, 医学研究科, 講師 (50360722)
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| Project Period (FY) |
2009 – 2010
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| Keywords | シグナル伝達 / 細胞・組織 / 一次繊毛 / 遺伝子 |
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| Research Abstract |
Primary cilia have diverse functions in cell signaling pathways, including mechanosensation and hedgehog signaling. More than 650 proteins are now identified to localize in the cilium. Inv, which is a mouse homolog of human Nphp2, is responsible for situs inversus and multiple renal cysts of the inv mutant mouse. Inv is localized at a distinctive proximal segment of the primary cilium, which we have named as the Inv compartment of the cilium. Nphp3 and Nek8/Nphp9 are also localized to the Inv compartment in wild type cells. Interestingly, the compartment localization of Nphp3 and Nek8/Nphp9 are lost in Inv mutant cells. Introduction of an Inv gene into Inv mutant cells restores in the compartment localization, suggesting that the compartment localization of Nphp3 and Nek8/Nphp9 is dependent on Inv function.
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