2010 Fiscal Year Final Research Report
Chloride dynamics in beating cardiomyocytes
| Project/Area Number |
21790200
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKEUCHI Ayako Kyoto University, 医学研究科, 助教 (00378704)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 心筋細胞 / 包括的心筋細胞モデル / 細胞内Cl^-動態 |
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| Research Abstract |
The purpose of the present study was to clarify the mechanisms and significance of Cl^-dynamics via intracellular organelle membranes in beating cardiomyocytes. To achieve these goals, cell physiological experiments in combination with computer simulation were performed and the following results were obtained. 1. Technique to measure the intracellular Cl^- dynamics was established. 2. Expression of CLIC2, an intracellular chloride channel, was analyzed in cell line of beating cardiomyocytes. 3. A comprehensive cardiomyocyte model was updated by incorporating pH homeostasis and detailed mitochondria to analyze dynamics of ions and energy substrates across the intracellular organelle membranes.
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