2010 Fiscal Year Final Research Report
Development of FRET probe for detection of G protein activity.
| Project/Area Number |
21790239
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MASUDA Kazuyuki The University of Tokyo, 先端科学技術研究センター, 助教 (20345151)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 受容体 / チャネル / 輸送系 / シグナル伝達 / RGS |
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| Research Abstract |
The purpose of this study is to develop FRET probe for detecting the activation of heterotrimeric G protein in living cells. The developed FRET probe couples to GPCR like as wild type G protein, shows ligand-induced FRET signal. The leukemia cell line expressed the FRET probe shows membrane protrusion subsequently to G protein activation during chemotaxis.
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