2010 Fiscal Year Final Research Report

Establishment of KRAP-transgenic mice and elucidation of KRAP function in cancer

Research Project

Project/Area Number	21790331
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Pathological medical chemistry
Research Institution	Fukuoka University
Principal Investigator	TAKAHIRO Fujimoto Fukuoka University, 医学部, 講師 (10446114)
Project Period (FY)	2009 – 2010
Keywords	KRAP / 結合蛋白 / 癌
Research Abstract	We generated KRAP-transgenic (TG) mice and identified intracellular signal-related molecule (X) as an associating protein of KRAP by using the TG-liver homogenate. KRAP colocalized with X molecule in both cultured cancer cell lines and mouse physiological tissues. Furthermore, the molecular association between KRAP and X molecules was validated by biochemical analyses. Finally, suppression of KRAP expression in living cells inhibited X-mediated signal transduction. These results showed that KRAP contributes to the regulation of intracellular signal transduction via physical association with X molecule.

(3 results)