2010 Fiscal Year Final Research Report
The cytotoxic mechanism of Vibiro parahaemolytiucs thermostable direct hemolysin and its application to a probe for lipid rafts
| Project/Area Number |
21790420
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUDA Shigeaki Osaka University, 微生物病研究所, 特任研究員 (30506499)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 腸炎ビブリオ / 耐熱性溶血毒 / 細胞傷害性 / 脂質ラフト |
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| Research Abstract |
Vibrio parahaemolyticus thermostable direct hemolysin (TDH) is associated with lipid rafts of cultured cells. This study addressed whether internalization of TDH correlates with its cytotoxicity. Inhibition of endocytosis did not affect TDH cytotoxicity for HeLa cells, indicating that internalization of TDH is not involved in its cytotoxicity. In contrast to cytotoxicity, hemolysis by TDH was independent of lipid rafts, suggesting that the mechanism of cytotoxicity by TDH appears to differ from that of hemolysis by TDH. Fluorescent dye-conjugated TDH was also constructed. This conjugate retained the cytotoxity and was associated with lipid rafts.
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