2011 Fiscal Year Final Research Report
Roles of lipid-recognition molecules in autoimmune diseases
| Project/Area Number |
21790463
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | University of Toyama |
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Principal Investigator |
NAGAI Yoshinori 富山大学, 大学院・医学薬学研究部(医学), 准教授 (30431761)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 免疫学 / 脂質 / 自己免疫病 / 自然免疫 |
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| Research Abstract |
In this study, we demonstrated that levels of soluble MD-1 markedly increased with disease progression in sera from MRL/lpr mice. To identify potential sources of serum soluble MD-1 in MRL/lpr mice, we measured the expression of MD-1 mRNA in the spleen, liver and kidney. In the liver and kidney of MRL/lpr mice, levels of MD-1 mRNA increased with age. Furthermore, the expression levels of MD-1 protein in the kidney of MRL/lpr mice increased with age. Finally, we investigated the cell types responsible for MD-1 expression in the kidney of MRL/lpr mice by immunohistochemical analyses. H & E staining of kidney sections revealed that Mac-2-positive macrophages accumulated in the perivascular lesions even at an early stage. More accumulated macrophages were observed in kidneys of 24-week old MRL/lpr mice than 8-week old ones. These cells were also stained with anti-MD-1 polyclonal antibody. These results suggest that macrophages in the kidney express MD-1 and might be a source of serum soluble MD-1 in MRL/lpr mice.
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