2011 Fiscal Year Final Research Report
The role of Wnt-5a as the pathological marker of inflammatory bowel disease.
| Project/Area Number |
21790688
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | Wnt-5a / 潰瘍性大腸炎 / クローン病 / myofibroblast / Hsp25 |
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| Research Abstract |
Colonic myofibroblast cells(VUPF) conditioned medium inhibited butyrate-induced Hsp25 expression and promoted butyrate induced cell proliferation. Mass spectroscopy of the active CM fraction implicated members of the Wnt-5a. Wnt5a expression was silenced in vivo by intramural injection with Wnt5a siRNA. At the site of injection, Hsp25 expression was increased along the entire length of the crypt in contrast to being only expressed by surface colonocytes in mice injected with scrambled siRNA. These findings were associated with decreased pericrypt MF Wnt-5a expression and decreased epithelial Ki-67 staining. When colonic crypt Hsp25 expression was increased through luminal lentiviral infection, Ki-67 staining was nearly absent. Wnt-5a mRNA expression of colonic mucosa from ulcerative colitis patients were significantly increased compated to normal control and remission stage of ulcerative colitis.
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