2011 Fiscal Year Final Research Report

Elucidation of mechanisms of peroxisomal disorders using pluripotent stem cells

Research Project

Project/Area Number	21790977
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Pediatrics
Research Institution	Gifu University
Principal Investigator	NAGASE Tomoko 岐阜大学, 生命科学総合研究支援センター, 助教 (20397326)
Project Period (FY)	2009 – 2011
Keywords	小児神経学
Research Abstract	To reveal the involvements of peroxisome during early developmental stage and obtain some clues as to the underlying cause of peroxisome biogenesis disorders(PBD_s), we performed neural differentiation studies using mouse embryonic stem(mES) cells and human induced pluripotent stem(iPS) cells. Few punctate staining patterns of catalase were observed in neural precursor cells and oligodendrocyte precursor cells differentiated from mES cells. Furthermore, we established iPS cells from fibroblasts derived from PBD patients, and performed neural differentiation studies using SDIA method. There is no obvious difference in efficiency of neural differentiation between a normal healthy individual and PBD patients.

(3 results)