2011 Fiscal Year Final Research Report
Systematic analysis of cornification related molecules and development of new treatments for skin disorders.
| Project/Area Number |
21791066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | University of Toyama |
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Principal Investigator |
MAKINO Teruhiko 富山大学, 大学院・医学薬学研究部(医学), 准教授 (90359711)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 皮膚生理学 |
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| Research Abstract |
The Profilaggrin N-terminal(proFLG-N) consists of two distinct domains, an S100-like A-domain and B domain. After proFLG-N transfection at a growing phase of NHK, only proFLG-N expressing cells exhibited DNA degradation. Therefore, A-domain in proFLG-N is responsible for DNA degradation. These results indicate that proFLG-N play an important role in the keratinocyte terminal differentiation, especially in the denucleation process. On the other hand, we identified novel S100 fused-type proteins, filaggrin-2(FLG2) and trichohyalin-like protein 1(TCHHL1). The structural features and expression profile of FLG2 were similar to those of profilaggrin. The deduced amino acid sequence of TCHHL1 contains one trans-membrane domain. The TCHHL1 protein was expressed in the basal layer.
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