n vivo imaging of bone and soft tissue tumor using fluorescent antibody

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: HAYASHI Katsuhiro Kanazawa University, 附属病院, 助教 (80507054)
• Project Period (FY): 2009 – 2010
• Keywords: 骨・軟部腫瘍学 / 蛍光イメージング

Research Abstract

We have investigated in vivo imaging of bone and soft tissue tumor using fluorescent antibody. Osteosarcoma cells were stained with fluorescent anti-ALP antibody. For distinction of normal tissue, red or near-infrared wavelength was suitable. Osteosarcoma cells were transplanted into mice and ICG was intravenously administered. Tumor was visualized by ICG. It can be used for tumor resection guide. Further investigation would be considered, such as improvement of observation devices.

Research Products

• [Journal Article] 転移リアルタイムイメージング (2010)
  • Author(s): 林克洋
  • Journal Title: 臨床整外 45 Pages: 1134-1138
  • Peer Reviewed
• [Journal Article] Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium. (2010)
  • Author(s): Kimura H, Zhang L, Zhao M, K Hayashi, Tsuchiya H, Tomita K, Bouvet M, Wessels J, Hoffman RM.
  • Journal Title: Cell Prolif 43 Pages: 41-48
  • Peer Reviewed
• [Journal Article] UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo. (2010)
  • Author(s): Kimura H, Lee C,K Hayashi, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Bouvet M, Hoffman RM.
  • Journal Title: J Cell Biochem 110 Pages: 1439-1446
  • Peer Reviewed
• [Journal Article] Real-time imaging of single cancer-cell dynamics of lung metastasis. (2010)
  • Author(s): Kimura H,K Hayashi, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM.
  • Journal Title: J Cell Biochem 109 Pages: 58-64
  • Peer Reviewed
• [Journal Article] Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model. (2010)
  • Author(s): Hasegawa A,K Hayashi, Kishimoto H, Yang M, Tofukuji S, Suzuki K, Nakajima H, Hoffman RM, Shirai M, Nakayama T.
  • Journal Title: J Allergy Clin Immunol 125 Pages: 461-468 e6
  • Peer Reviewed
• [Journal Article] A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis. (2009)
  • Author(s): Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Bouvet M, Wessels J, Hoffman RM.
  • Journal Title: Cell Prolif 42 Pages: 75-82
  • Peer Reviewed
• [Journal Article] Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhimurium. (2009)
  • Author(s): Hayashi K, Zhao M, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Hoffman RM.
  • Journal Title: J Cell Biochem 106 Pages: 992-998
  • Peer Reviewed
• [Journal Article] In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. (2009)
  • Author(s): Tome Y, Tsuchiya H,K Hayashi, Yamauchi K, Sugimoto N, Kanaya F, Tomita K, Hoffman RM.
  • Journal Title: J Cell Biochem 108 Pages: 362-367
  • Peer Reviewed
• [Presentation] Leading technology for in vivo fluorescent sarcoma imaging. (2011)
  • Author(s): Katsuhiro Hayashi, Norio Yamamoto, Toshiharu Shirai, Hideji Nishida, Akihiko Takeuchi, Hiroaki Kimura, Robert M Hoffman, Hiroyuki Tsuchiya.
  • Organizer: AAOS American Academy of Orthopaedic Surgeons
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 20110215-20110219
• [Presentation] サルモネラ変異株を応用したリンパ節転移の治療と蛍光イメージング (2010)
  • Author(s): 林克洋、土屋弘行
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2010-10-14
• [Presentation] Targeting of primary tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. (2010)
  • Author(s): Hayashi K, Tsuchiya H.
  • Organizer: 日本整形外科学会学術総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2010-05-27
• [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング (2009)
  • Author(s): 林克洋, 土屋弘行, 山本憲男, 白井寿治, 山内健輔, ロバートホフマン, 富田勝郎
  • Organizer: 第18回日本がん転移学会学術集会・総会
  • Place of Presentation: 旭川グランドホテル(北海道)
  • Year and Date: 20090723-20090724
• [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング (2009)
  • Author(s): 林克洋、土屋弘行
  • Organizer: 第42回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 20090717-20090718
• [Presentation] Dual color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. (2009)
  • Author(s): 林克洋、土屋弘行
  • Organizer: 第52回日本整形外科学会学術総会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 20090514-20090517
• [Presentation] Real Time Imaging of Cancer Cell Seeding to the Lung in Live Mouse. (2009)
  • Author(s): K Hayashi, H Tsuchiya, N Yamamoto, T Shirai, H Nishida, K Yamauchi, A Takeuchi, H Kimura, K Tomita, M Bouvet, RM.Hoffman.
  • Organizer: 100th American Association for Cancer Research
  • Place of Presentation: Colorado Convention Center (USA)
  • Year and Date: 20090412-20090416