2010 Fiscal Year Final Research Report
The effect of TS-1 and enhanced chemosensitivity to TS-1 by rTS gene therapy in bladder cancer.
| Project/Area Number |
21791529
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Keio University |
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Principal Investigator |
IDE Hiroki Keio University, 医学部, 助教 (70365360)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 膀胱癌 / 化学療法 / 遺伝子治療 |
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| Research Abstract |
This experiments revealed that the treatment with TS-1 resulted in a significantly higher level of growth inhibition compared to that with other 5-FU-based agent in bladder cancer cell lines with a higher level of DPD. Furthermore, our study showed that bladder cancer cells with higher TS or DPD have lower sensitivity of 5-FU and bladder cancer cells in which the levels of TS and DPD were down-regulated using siRNA had higher sensitivity of 5-FU. Thus, these results indicated that elevated TS and DPD levels were strongly associated with decreased sensitivity of 5-FU in bladder cancer.
The present study showed that the treatment of TS-1 in combination with CPT-11 that was able to down-regulate the level of TS resulted in a significantly higher level of growth inhibition compared to that with TS-1 alone or CPT-11 alone. Thus, from these results, the combination treatment of TS-1 in combination with rTS gene therapy in which the level of TS was able to be down-regulated might be a novel treatment against bladder cancer.
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