バクテロデスフィラムに位置する細菌の菌体外蛋白質分泌機構の解明

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791793
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SATO Keiko Nagasaki University, 医歯薬学総合研究科, 助教 (70410579)
• Project Period (FY): 2009 – 2010

Research Abstract

Porphyromonas gingivalis is an oral anaerobic Gram-negative bacterium, which is implicated in periodontal pathogenesis. Most of proteolytic activity of extracellular and cell-surface proteinase are derived from cysteine proteinase named gingipains. We found a novel protein secretion system in bacteria of the phylum Bacteroidetes, which is responsible for secretion of virulence factors such as gingipain proteinases produced from the periodontal pathogen Porphyromonas gingivalis and is linked to bacteroidete gliding motility that is distinct from other well-studied forms of bacterial movement.

Research Products

• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis.(共著) (2011)
  • Author(s): Yukitake H, Naito M, Sato K, Shoji M, Ohara N, Yoshimura M, Sakai E, Nakayama K
  • Journal Title: Microbiology and Immunology 55巻3号 Pages: 141-153
• [Journal Article] Porphyromonas gingivalis mutant defective in a putative glycosyltransferase exhibits defective biosynthesis of polysaccharide portions of lipopolysaccharide decreased gingipain activities, strong autoaggregation and increased biofilm formation.(共著) (2010)
  • Author(s): Yamaguchi M, Sato K, Yukitake H, Noiri Y, Ebisu S, Nakayama K
  • Journal Title: Infection and Immunity 78巻9号 Pages: 3801-3812
• [Journal Article] Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis.(共著) (2010)
  • Author(s): Kondo Y, Ohara N, Sato K, Yoshimura M, Yukitake H, Naito M, Fujiwara T, Nakayama K
  • Journal Title: Infection and Immunity 78巻6号 Pages: 2846-2856
• [Journal Article] Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization(共著) (2010)
  • Author(s): hoji M, Shibata Y, Shiroza T, Yukitake H, Peng B, Chen Y-Y, Sato K, Naito M, Abiko Y, Reynolds EC, Nakayama K
  • Journal Title: BMC Microbiology 10巻 Pages: 152
• [Journal Article] A protein secretion system linked to bacteroidete gliding motility and pathogenesis.(共著) (2010)
  • Author(s): Sato K, Naito M, Yukitake H, Hirakawa H, Shoji M, McBride MJ, Rhodes RG, Nakayama K
  • Journal Title: Proc.Natl.Acad.Sci.USA 107巻 Pages: 276-281
• [Journal Article] Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2.(共著) (2009)
  • Author(s): Hasegawa Y, Iwami J, Sato K, Park Y, Nishikawa K, Atsumi T, Moriguchi K, Murakami Y, Yoshimura F
  • Journal Title: Microbiology. 155巻10号 Pages: 3333-3347
• [Presentation] バクテロイデーテス細菌におけるタンパク分泌、滑走運動および病原性 (2010)
  • Author(s): 佐藤啓子、内藤真理子、雪竹英治、庄子幹郎、中山浩次
  • Organizer: 第52回歯科基礎医学会(学術大会ならびに総会)
  • Year and Date: 20100900
• [Presentation] Gliding and Secretion of Flavobacterium johnsoniae (2010)
  • Author(s): 佐藤啓子、雪竹英治、内藤真理子、庄子幹郎、中山浩次
  • Organizer: 第83回日本細菌学会総会
  • Year and Date: 20100300
• [Remarks] ホームページ等