Novel function of the signaling molecule, PRIP.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791807
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: TAKEUCHI Hiroshi Kyushu University, 大学院・歯学研究院, 准教授 (70304813)
• Project Period (FY): 2009 – 2010
• Keywords: シグナル伝達 / 脂質 / 開口分泌

Research Abstract

In this study, we examined the role of PRIP in regulated exocytosis, the universal cellular process through which various hormones, neurotransmitters and cytokines are secreted. We discovered that PRIP negatively regulates exocytosis by competing for membrane phospholipid, PtdIns(4,5)P_2 with a Ca^<2+>-dependent priming factor, CAPS. We also found that PRIP can also inhibit exocytosis independent of its PtdIns(4,5)P_2-binding ability.

Research Products

• [Journal Article] Characterization of PXK as a protein involved in epidermal growth factor receptor trafficking. (2010)
  • Author(s): Takeuchi, H., Takeuchi, T., Gao, J., Cantley, L.C., Hirata, M.
  • Journal Title: Mol.Cell.Biol. 30 Pages: 1689-1702
• [Journal Article] Assay of dense-core vesicle exocytosis using permeabilized PC12 cells. (2010)
  • Author(s): Gao, J., Takeuchi, H., Umebayashi, H., Zhang, Z., Matsuda, M., Hirata, M.
  • Journal Title: Adv.Enzyme Regul. 50 Pages: 237-246
• [Journal Article] Binding of phospholipase C-related but catalytically inactive protein to phosphatidylinositol 4, 5-bisphoshate via the PH domain. (2009)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Fujii, M., Kanematsu, T., Hirata, M.
  • Journal Title: Cell.Signal. 21 Pages: 1180-1186
• [Presentation] Contribution of the C2 domain to the binding of phospholipase C-related but catalytically inactive protein with SNARE proteins. (2010)
  • Author(s): Zhang, Z., Takeuchi, H., Gao, J., Hirata, M.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 20101207-20101210
• [Presentation] Implication of PRIP in phosphor-dependent regulation of exocytosis. (2010)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Hirata, M.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 20101207-20101210
• [Presentation] PRIP is involved in PtdIns(4,5)P2-dependent regulation of dense core vesicle exocytosis. (2010)
  • Author(s): Takeuchi, H., Gao, J., Zhang, Z., Sugiyama, G., Hirata, M.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 20101207-20101210
• [Presentation] タンパク質ホスファターゼ PP1 及び PP2A と PRIPの相互作用についての解析 (2010)
  • Author(s): 杉山悟郎、竹内弘、高靖、張〓、松田美穂、兼松隆、平田雅人
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 20101207-20101210
• [Presentation] PRIPはタンパク質ホスファターゼPP1及びPP2Aの機能を制御する (2010)
  • Author(s): 杉山悟郎、竹内弘、松田美穂、兼松隆、平田雅人
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 20100920-20100922
• [Presentation] 新しいソーティングネキシン (2010)
  • Author(s): 竹内弘
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 20100920-20100922
• [Presentation] Finding of a new protein named PRIP, and struggling to explore the biological function.International Symposium, Network for International Education and Research in Advanced Dental Sciences. (2010)
  • Author(s): Hirata, M., Gao, J., Matsuda, M., Takeuchi, H., Kanematsu, T.
  • Organizer: Niigata
  • Year and Date: 20100209-20100210
• [Presentation] PRIP, a new Ins(1,4,5)P3 binding protein, its extension to neuroscience and beyond. (2010)
  • Author(s): Hirata, M., Gao, J., Matsuda, M., Takeuchi, H., Kanematsu, T.
  • Organizer: 2nd Pohang Conference on Cellular Signaling.
  • Place of Presentation: Pohang, Korea
  • Year and Date: 20100202-20100203
• [Presentation] Binding of phospholipase C-related but catalytically inactive protein to SNARE proteins. (2010)
  • Author(s): Zhang, Z., Takeuchi, H., Gao, J., Hirata, M.
  • Organizer: OzBio2010
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-09-29
• [Presentation] Regulatory role of PRIP in exocytosis through the interaction with protein phosphatase. (2010)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Hirata, M.
  • Organizer: OzBio2010
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-09-27
• [Presentation] Roles for PRIP in dense core vesicle exocytosis. (2010)
  • Author(s): Gao, J., Takeuchi, H., Hirata, M.
  • Organizer: The 5th International Symposium on Oral Health Science in 2010
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-02-06
• [Presentation] Pleckstrin homology domain of PRIP is implicated in the inhibition of dense-core vesicle secretion in PC 12 cells. (2009)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Hirata, M.
  • Organizer: The 6th Japan-Korea Conference on Cellular Signaling for Young Scientists.
  • Place of Presentation: Sasebo
  • Year and Date: 20091124-20091126
• [Presentation] Inhibition of exocytosis by PRIP, phospholipase C-related inactive protein. (2009)
  • Author(s): Hirata, M., Gao, J., Takeuchi, H.
  • Organizer: The 50th International Symposium on "Regulation of Enzyme Activity and Synthesis in Normal and Neoplastic Tissues".
  • Place of Presentation: Bologna, Italy
  • Year and Date: 20090928-20090929
• [Presentation] The role of C2 domain of PRIP in dense core vesicle exocytosis. (2009)
  • Author(s): Zhang, Z., Takeuchi, H., Gao, J., Hirata, M.
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology.
  • Place of Presentation: Shanghai, China
  • Year and Date: 20090802-20090807
• [Presentation] Pleckstrin homology domain of PRIP inhibits exocytosis in PC12 cells by binding to phosphatidylinositol 4,5-bisphosphate. (2009)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Kanematsu, T., Hirata, M.
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology.
  • Place of Presentation: Shanghai, China
  • Year and Date: 20090802-20090807
• [Presentation] Inhibitory role of PRIP in dense core vesicle exocytosis. (2009)
  • Author(s): Takeuchi, H., Gao, J., Zhang, Z., Hirata, M.
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology.
  • Place of Presentation: Shanghai, China
  • Year and Date: 20090802-20090807
• [Presentation] Pleckstrin homology domain of phospholipase C-related but catalytically inactive protein inhibits the dense-core vesicle secretion in PC12 cells. (2009)
  • Author(s): 高靖、竹内弘、張〓、平田雅人
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-10-23
• [Presentation] Phospholipase C-related but catalytically inactive protein is implicated in the inhibition of dense-core vesicle secretion in PC12 cells. (2008)
  • Author(s): Gao, J., Takeuchi, H., Kanematsu, T., Hirata, M.
  • Organizer: 先端歯学スクール 2009
  • Place of Presentation: 淡路市
  • Year and Date: 20080827-20090828
• [Remarks] ホームページ
  • URL: http://www.dent.kyushu-u.ac.jp/sosiki/a04/index.html