2010 Fiscal Year Final Research Report
An investigation of tissue regeneration therapy using dental pulp-derived CD45(-), Sca-1(+) and CXCR4(+) cells transplantation.
| Project/Area Number |
21791958
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kansai Medical University |
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Principal Investigator |
NAKATSUKA Ryusuke Kansai Medical University, 医学部, 助教 (90454561)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 歯髄幹細胞 / 分化可塑性 |
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| Research Abstract |
In this study, we investigated whether these DPSCs can be prospectively isolated by FACS using bone marrow-derived mesenchymal stem cell (MSC) markers. Furthermore, characteristics of these DPSCs have been extensively investigated using FACS, immunostaining, and in vitro tissue-orientetd differentiation cultures. This study developed a novel method to prospectively isolate the dental pulp-derived mouse DPSCs and revealed that the identified DPSCs possess the potential to differentiate into multiple lineages similar to the MSCs, and these cells exist in the niche for tooth forming cells.
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[Journal Article] Development of a High Resolution Purification Method for Precise Functional Characterization of Primitive Human Cord Blood-derived CD34-negative SCID-repopulating Cells.2011
Author(s)
Ishii M, Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Asano H, Uemura Y, Tsuji T, Fukuhara S, Sonoda Y.
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Journal Title
Experimental hematology. 39(2)
Pages: 203-213
Peer Reviewed
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[Journal Article] CXCL8 enhances the angiogenic activity of umbilical cord blood-derived outgrowth endothelial cells in vitro.2011
Author(s)
Kimura T, Kohno H, Matsuoka Y, Murakami M, Nakatsuka R, Hase M, Yasuda K, Uemura Y, Sasaki Y, Fukuhara S, Sonoda Y.
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Journal Title
Cell biology international. 35(3)
Pages: 201-208
Peer Reviewed
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[Journal Article] In vivo dynamics of human cord blood-derived CD34(-) SCID-repopulating cells using intra-bone marrow injection.2010
Author(s)
Kimura T, Matsuoka Y, Murakami M, Kimura T, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Kobayashi K, Imai S, Asano H, Nakatsuka R, Uemura Y, Sasaki Y, Sonoda Y.
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Journal Title
Leukemia. 24(1)
Pages: 162-168
Peer Reviewed
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[Journal Article] Cytokine-dependent modification of IL-12p70 and IL-23 balance in dendritic cells by ligand activation of Valpha24 invariant NKT cells.2009
Author(s)
Uemura Y, Liu TY, Narita Y, Suzuki M, Nakatsuka R, Araki T, Matsumoto M, Iwai LK, Hirosawa N, Matsuoka Y, Murakami M, Kimura T, Hase M, Kohno H, Sasaki Y, Ichihara Y, Ishihara O, Kikuchi H, Sakamoto Y, Jiao SC, Senju S, Sonoda Y.
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Journal Title
Journal of Immunology. 183(1)
Pages: 201-208
Peer Reviewed
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