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2010 Fiscal Year Final Research Report

Real-time monitoring of single biomolecular interaction under microfluidic control

Research Project

  • PDF
Project/Area Number 21810012
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionNagoya University

Principal Investigator

ONOSHIMA Daisuke  Nagoya University, 工学研究科, 研究員 (40510219)

Project Period (FY) 2009 – 2010
Keywords1分子計測(SMD) / マイクロ・ナノデバイス / ナノバイオ / 生物物理 / 生体分子
Research Abstract

Single-molecule, real-time intermolecular interaction analysis was achieved by microfluidic control. The method enabled tracking and analysis of biomolecules in a physiologically natural state. Further applications of our method will provide new understanding of molecular mechanisms by which DNA and protein perform their diverse biological functions in cells.

  • Research Products

    (17 results)

All 2011 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (12 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Tracking Degradations of Single DNA and Protein Molecules in Fluid2011

    • Author(s)
      D.Onoshima, N.Kaji, M.Tokeshi, Y.Baba
    • Journal Title

      Biophysical Journal Vol.100

      Pages: 151a-152a

    • Peer Reviewed
  • [Journal Article] TRACKING OF SINGLE DNA AND PROTEIN MOLECULES UNDERGOING ENZYMATIC DEGRADATION IN FLUID2010

    • Author(s)
      D.Onoshima, N.Kaji, M.Tokeshi, Y.Baba
    • Journal Title

      Proceedings of μTAS 2010 Vol.1

      Pages: 1439-1441

    • Peer Reviewed
  • [Journal Article] A SIMPLE AND LOW-COST TECHNIQUE FOR FABRICATING MICROCHANNELS AND MICROSTRUCTURES WITH HIGH ASPECT RATIO2009

    • Author(s)
      D.Onoshima, J.Wang, M.Aki, K.Arinaga, N.Kaji, M.Tokeshi, S.Fujita, N.Yokoyama, Y.Baba
    • Journal Title

      The Proceedings of μTAS 2009 Vol.2

      Pages: 1587-1589

    • Peer Reviewed
  • [Presentation] マイクロ流体制御下におけるプロテアーゼ反応の分子的検出2011

    • Author(s)
      小野島大介・加地範匡・渡慶次学・馬場嘉信
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      講演予稿集
    • Year and Date
      2011-03-11
  • [Presentation] Tracking Degradations of Single DNA and Protein Molecules in Fluid2011

    • Author(s)
      小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
    • Organizer
      Biophysical Society 55th Annual Meeting
    • Place of Presentation
      Baltimore Convention Center, Baltimore, Maryland, USA
    • Year and Date
      2011-03-06
  • [Presentation] マイクロ流体デバイスを利用した1分子生物学への新しいアプローチ、4大学ナノ・マイクロファブリケーションコンソーシアム 拠点形成シンポジウム2011

    • Author(s)
      小野島大介
    • Organizer
      「グリーンイノベーション・ライフイノベーションを促進する 材料、計測、ファブリケーション、デバイス技術
    • Place of Presentation
      川崎市産業振興会館 1F メインホール
    • Year and Date
      2011-03-04
  • [Presentation] Optical Tracking of Single Biomolecules Undergoing EnzymaticDegradations2011

    • Author(s)
      小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
    • Organizer
      InternationalSymposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules(ISSMA2011)
    • Place of Presentation
      Kyoto International Conference Center (ICC Kyoto), Kyoto, Japan
    • Year and Date
      2011-01-24
  • [Presentation] A molecular tracking ofprotein-DNA interactions under microfluidic control2010

    • Author(s)
      小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
    • Organizer
      The International Chemical Congress ofPacific Basin Societies(Pacifichem2010)
    • Place of Presentation
      Hawaii Convention Center, Honolulu, Hawaii, USA
    • Year and Date
      2010-12-15
  • [Presentation] プロテアーゼ反応の分子的リアルタイムトラッキング2010

    • Author(s)
      小野島大介・加地範匡・渡慶次学・馬場嘉信
    • Organizer
      第22回化学とマイクロ・ナノシステム研究会(22nd CHEMINAS)
    • Place of Presentation
      名古屋大学鶴舞キャンパス
    • Year and Date
      2010-11-18
  • [Presentation] TRACKING OF SINGLE DNA AND PROTEIN MOLECULES UNDERGOING ENZYMATIC DEGRADATION IN FLUID2010

    • Author(s)
      小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
    • Organizer
      The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010)
    • Place of Presentation
      Martiniplaza, Groningen, Nertherlands
    • Year and Date
      2010-10-06
  • [Presentation] A molecular detection of nucleolytic degradation under microfluidic control2010

    • Author(s)
      小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
    • Organizer
      International Symposium on Microchemistry and Microsystems 2010 (ISMM 2010)
    • Place of Presentation
      Hong Kong University of Science and Technology (HKUST), Hong Kong
    • Year and Date
      2010-05-28
  • [Presentation] マイクロ流体制御下における核酸分解酵素反応の分子的検出2010

    • Author(s)
      小野島大介、加地範匡、渡慶次学、馬場嘉信
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2010-03-27
  • [Presentation] A Combination of a Single-Molecule Tracking and Microfluidic Flow System Enables an Analysis of Ongoing Processes of Enzymatic Reaction2009

    • Author(s)
      小野島大介
    • Organizer
      BIT's World Congress of Gene-2009 (WCG-2009)
    • Place of Presentation
      Foshan Golden Sun Hotel (FGSH), Foshan, China
    • Year and Date
      2009-12-05
  • [Presentation] ヌクレアーゼ相互作用のマイクロ流体制御と1分子リアルタイム計測2009

    • Author(s)
      小野島大介・加地範匡・渡慶次学・馬場嘉信
    • Organizer
      第40回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      岐阜大学工学部
    • Year and Date
      2009-11-08
  • [Presentation] A SIMPLE AND LOW-COST TECHNIQUE FOR FABRICATING MICROCHANNELS AND MICROSTRUCTURES WITH HIGH ASPECT RATIO2009

    • Author(s)
      D.Onoshima, J.Wang, M.Aki, K.Arinaga, 加地範匡, 渡慶次学, S.Fujita, N.Yokoyama, 馬場嘉信
    • Organizer
      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2009)
    • Place of Presentation
      International Convention Center at Jeju Istand (ICC Jeju), Korea
    • Year and Date
      2009-11-04
  • [Book] 『化学フロンティア22生命現象を理解する分子ツール イメージングから生体機能解析まで』IV部19章(浜地格・二木史朗編)2010

    • Author(s)
      小野島大介・馬場嘉信
    • Total Pages
      155-161
    • Publisher
      化学同人
  • [Patent(Industrial Property Rights)] 分析デバイス、分析デバイスの製造方法及び分析デバイスを用いる分析装置2009

    • Inventor(s)
      小野島大介・加地範匡・渡慶次学・馬場嘉信
    • Industrial Property Rights Holder
      名古屋大学
    • Industrial Property Number
      特許,特願2009-251536
    • Filing Date
      2009-10-30

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Published: 2012-01-26   Modified: 2016-04-21  

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