2010 Fiscal Year Final Research Report
Development of live-cell imaging for photosystem proteins by using the Physcomitrella patens giant chloroplasts
| Project/Area Number |
21870047
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
IWAI Masakazu The Institute of Physical and Chemical Research, ライブセル分子イメージング研究チーム, 基礎科学特別研究員 (80553768)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ライブセルイメージング / 光合成研究 / 巨大葉緑体 / ヒメツリガネゴケ / 集光アンテナタンパク質 / 光環境適応機構 |
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| Research Abstract |
We developed the imaging technique using a confocal microscopy to observed photosynthetic proteins in live plant cells. Using a moss Physcomitrella patens, whose rate of homologous recombination of the nuclear genes is extremely high, we fused a green fluorescent protein (GFP) to the C-terminal region of LHCB9, a moss specific light-harvesting antenna protein. As a result, we visualized the LHCB9-GFP in the giant chloroplast thylakoid membranes in live plant cells for the first time. The results obtained here will be useful for the visualization of other important proteins related to the light energy transfer in chloroplast thylakoid membranes.
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