Molecular mechanism of trans-translation

2010 Fiscal Year Final Research Report

• Project/Area Number: 21880009
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Hirosaki University
• Principal Investigator: KURITA Daisuke 弘前大学, 農学生命科学部, 助教 (60552651)
• Project Period (FY): 2009 – 2010
• Keywords: tmRNA,SmpB,リボソーム / トランス翻訳

Research Abstract

To reveal the molecular mechanism of trans-translation, we developed in vitro trans-translation system using purified factors. Using this system, we showed that the C-terminal domain of SmpB plays a role in accommodation of tmRNA into the ribosomal A-site.

Research Products

• [Journal Article] tRNA/mRNA mimicry by tmRNA and SmpB in trans-translation (2011)
  • Author(s): Kurita D, Muto A, Himeno H
  • Journal Title: Journal of Nucleic Acids
• [Journal Article] Trans-translation by tmRNA and SmpB: a bacterial quality control system of translation (2010)
  • Author(s): Himeno H, Kurita D, Muto, A
  • Journal Title: Advances in Genetics Research
• [Journal Article] 2つのtRNA/mRNAハイブリッド-トランス・トランスレーション-mRNAプログラム 多様性と非対称性の獲得戦略(稲田利文、大野睦人編) (2010)
  • Author(s): 姫野俵太、栗田大輔、武藤昇
  • Journal Title: 共立出版 Pages: 2201-2206
• [Journal Article] Role of the C-terminal tail of SmpB in the early stage of trans-translation (2010)
  • Author(s): Kurita D, Muto A, Himeno H
  • Journal Title: RNA Volume: 5 Pages: 980-990
• [Journal Article] Trans-translation by tmRNA and SmpB (2009)
  • Author(s): Une M, Kurita D, Muto A, Himeno H
  • Journal Title: Nucleic Acids Symp Ser Volume: 53 Pages: 305-306
• [Journal Article] 2つのtRNA/mRNAハイブリッド-トランス・トランスレーション-蛋白質核酸酵素 (2009)
  • Author(s): 姫野俵太、栗田大輔、武藤昇
  • Journal Title: 共立出版 Pages: 2201-2206
• [Presentation] Molecular mechanism of trans-translation mediated by tmRNA/SmpB (2011)
  • Author(s): Himeno H, Kurita D, Muto A
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110900
• [Presentation] Molecular mechanism of the early stages of trans-translation by tmRNA/SmpB (2011)
  • Author(s): Kurita D, Hattori Y, Muto A, Himeno H
  • Organizer: 16th Annual meeting of the RNA society
  • Place of Presentation: Kyoto
  • Year and Date: 20110600
• [Presentation] tRNA/mRNAハイブリッドとtRNA/mRNA擬態タンパク質によるtrans-translation (2010)
  • Author(s): 姫野俵太、栗田大輔、宇根理高、武藤昇
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20101200
• [Presentation] Molecular mechanism of the early stage of trans-translation (2010)
  • Author(s): Kurita D, Une M, Muto A, Himeno H
  • Organizer: 7th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20101100
• [Presentation] Trans-translation by tmRNA/SmpB (2010)
  • Author(s): Kurita D, Muto A, Himeno H
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: Nara, Japan
  • Year and Date: 20100900
• [Presentation] 無細胞トランス・トランスレーション系におけるSmpBのC末端tailの働き (2010)
  • Author(s): 栗田大輔、武藤昇、姫野俵太
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山
  • Year and Date: 20100900
• [Presentation] トランス・トランスレーションの初期過程におけるSmpB C末端tailの働き (2010)
  • Author(s): 栗田大輔、宇根理高、武藤昇、姫野俵太
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 東京
  • Year and Date: 20100700
• [Presentation] リボソーム成熟因子RsgA/YjeQの構造機能解析 (2010)
  • Author(s): 竹本千重、Sean R. Connell、長谷要一、直枝智恵子、王宏飛、上西達也、菊池岳志、平田侑也、栗田大輔、武藤昇、武藤裕、横山茂之、Paola Fucini、姫野俵太
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 東京
  • Year and Date: 20100700
• [Presentation] Molecular mimicry by tmRNA/SmpB during trans-translation (2010)
  • Author(s): Kurita D, Une M, Muto A, Himeno H
  • Organizer: Ribosomes 2010 meeting
  • Place of Presentation: Orvieto, Italy
  • Year and Date: 20100500
• [Presentation] Structural studies for RsgA/YjeQ, a ribosome maturation factor (2010)
  • Author(s): Takemoto C, Connell SR, Hase Y, Naoe C, Wang H, Kaminishi T, Kikuchi T, Hirata Y, Kurita D, Muto A, Muto Y, Yokoyama S, Himeno H
  • Organizer: Ribosomes 2010 meeting
  • Place of Presentation: Orvieto, Italy
  • Year and Date: 20100500
• [Presentation] Molecular mimicry of tRNA/mRNA during trans-translation (2010)
  • Author(s): Kurita D, Une M, Muto A, Himeno H
  • Organizer: ASBMB 2010 Annual meeting
  • Place of Presentation: Anaheim, USA
  • Year and Date: 20100400
• [Presentation] Role of the C-terminal tail of SmpB during trans-translation (2010)
  • Author(s): Kurita D, Une M, Muto A, Himeno H
  • Organizer: 23rd tRNA workshop
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 20100100
• [Presentation] Trans-translation by tmRNA and SmpB (2009)
  • Author(s): Une M, Kurita D, Muto A, Himeno H
  • Organizer: 6th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Takayama, Gifu, Japan
  • Year and Date: 20090900
• [Presentation] 部位特異的ヒドロキシラジカルプロービングを用いたRsgAとリボソームの相互作用の解析 (2009)
  • Author(s): 菊地岳志、平田侑也、栗田大輔、長谷要一、木村天胤、武藤昇、姫野俵太
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
  • Year and Date: 20090700
• [Book] Methods in Molecular Biology
  • Author(s): Kurita D, Muto A, Himeno H
  • Publisher: In vitro trans-translation assays(In press)