2010 Fiscal Year Final Research Report

Epidemiological and molecular investigation on prevalence of antimicrobial resistance in Escherichia coli isolates from pups, diseased dogs, and humans.

Research Project

Project/Area Number	21880043
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Applied veterinary science
Research Institution	Nippon Veterinary and Life Science University
Principal Investigator	HARADA Kazuki Nippon Veterinary and Life Science University, 獣医学部, 助教 (80549543)
Co-Investigator(Renkei-kenkyūsha)	SAWADA Takuo 日本獣医生命科学大学, 名誉教授 (30226071) TAKAHASHI Toshio 日本獣医生命科学大学, 獣医学部, 教授 (90586273) KATAOKA Yasushi 日本獣医生命科学大学, 獣医学部, 准教授 (60277663)
Project Period (FY)	2009 – 2010
Keywords	大腸菌 / 薬剤耐性 / 犬 / ヒト / 病原因子 / クローン
Research Abstract	In this study, I investigated prevalence of antimicrobial resistance and their relative factors in Escherichia coli isolates from dogs and humans. Pup-origin isolates spread clonally in each kennel, resulting in different prevalence of resistance between kennels. In addition, resistance prevalence was associated with virulence factors and phylogenetic groups in dogs with urogenital infections. Furthermore, virulence factors, but not antimicrobial resistance, was more prevalent in canine isolates than in owner isolates, and genetic analysis revealed clone sharing between dogs and their owners.

(4 results)