2010 Fiscal Year Final Research Report
Development of functional oligosaccharides using glycoside hydrolases from basidiomycetes
| Project/Area Number |
21880050
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Iwate Biotechnology Research Center |
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Principal Investigator |
KONNO Naotake (財)岩手生物工学研究センター, 生物資源研究部, 研究員 (60549880)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAMOTO Yuichi 財団法人岩手生物工学研究センター, 生物資源研究部, 主任研究員 (80390889)
YAMADA Hidetoshi 財団法人岩手生物工学研究センター, 生物資源研究部, 研究員 (70511955)
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| Project Period (FY) |
2009 – 2010
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| Keywords | バイオマス / 糖質分解酵素 / 担子菌 / オリゴ糖 / 機能性食品 / シイタケ |
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| Research Abstract |
The cell walls of fungi are constructed mainly from chitin and β-1,3/1,6-glucan, and fungi are known to produce enzymes associated with cell-wall polysaccharides. In this study, we purified β-1,3- and β-1,6-glucanase, GLU1 and LePUS30A, from the fruiting body of Lentinula edodes (Shiitake mushroom). GLU1 specifically degraded β-1,3-glucans with an endo-type manner. LePUS30A catalyzed depolymerization of β-1,6-glucan endolytically, and the enzymatic products induced TNF α production.
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